Anti-phospho-STAT2 (Tyr689) Antibody

The Janus kinase (JAK)/signal transducers and activators of transcription (STAT) signaling pathway plays an important role in cell proliferation, cell differentiation, cell migration, and cell death. It is the principal signaling mechanism for a variety of cytokines and growth factors. Constitutive activation or dysregulation of JAK/STAT signaling can result in inflammatory disease, erythrocytosis, gigantism, and leukemia. STAT2 (113 kDa), originally purified from the nuclei of a-interferon-treated cells, is critical to the transcriptional responses induced by type I interferons, IFN-alpha/beta. Knockout mice with a targeted disruption of STAT2 exhibit greater susceptibility to viral infection and altered responses to Type I interferons. Stat2 is rapidly activated by phosphorylation at Tyr690 in response to stimulation by IFN-alpha/beta via assocations with receptor-bound Jak kinases.

Other homologies: Rat (89% sequence homology).

This antibody recognizes STAT2 phosphorylated at Tyr689.

Epitope: Phosphorylated Tyr689.

KLH-conjugated linear peptide corresponding to mouse STAT2 phosphorylated at Tyr689.

Anti-phospho-STAT2 (Tyr689) Antibody is an antibody against phospho-STAT2 (Tyr689) for use in Immunoprecipitation.

Immunoprecipitation Analysis: 10 µg from a representative lot immunoprecipitated phospho-STAT2 (Tyr689) from IFN alpha treated HeLa cell lysate.

Research Category
Epigenetics & Nuclear Function

Research Sub Category
Immunological Signaling

Evaluated by Western Blot in HeLa cell lysate.

Western Blot Analysis: A 1:200 dilution of this antibody detected phospho-STAT2 (Tyr689) in 10 µg of IFN alpha/gamma treated HeLa cell lysate.

~113 kDa observed. An uncharacterized band may appear at ~260 kDa in some lysates.

Affinity purified

Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Stable for 1 year at 2-8°C from date of receipt.

Control
Untreated and IFN alpha/gamma treated HeLa cell lysate.

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