ABE496
Anti-Lysine-specific demethylase 5C Antibody
from rabbit, purified by affinity chromatography
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Lysine-specific demethylase 5C, Histone demethylase JARID1C, Jumonji/ARID domain-containing protein 1C, Protein SmcX, Protein Xe169
Recommended Products
biological source
rabbit
Quality Level
antibody form
affinity isolated antibody
antibody product type
primary antibodies
clone
polyclonal
purified by
affinity chromatography
species reactivity
human
technique(s)
immunohistochemistry: suitable
immunoprecipitation (IP): suitable
western blot: suitable
NCBI accession no.
UniProt accession no.
shipped in
wet ice
target post-translational modification
unmodified
Gene Information
human ... KDM5C(8242)
General description
Lysine-specific demethylase 5C is also called Histone demethylase JARID1C, Jumonji/ARID domain-containing protein 1C, Protein SmcX and Protein Xe169. Lysine-specific demethylase 5C is a histone demethylase that specifically targets trimethylated and dimethylated, but not monomethylated, ′Lys-4′ of histone H3, making it a regulator of the histone code. Lysine-specific demethylase 5C is involved in transcriptional repression of neuronal genes by recruiting histone deacetylases and REST at neuron-restrictive silencer elements and is associated with Mental retardation, X-linked, syndromic, Claes-Jensen type (MRXSCJ). Lysine-specific demethylase 5C is widely expressed with highest levels found in brain and skeletal muscle.
Specificity
This antibody is expected to react with all four known isoforms based upon immunogen design.
Immunogen
KLH-conjugated linear peptide corresponding to a region near the near the N-terminus of human Lysine-specific demethylase 5C.
Application
Detect JARID using this rabbit polyclonal antibody, Anti-Lysine-specific demethylase 5C Antibody validated for use in western blotting, IP & IHC.
Immunoprecipitation Analysis: 10 µg of this antibody immunoprecipitated Lysine-specific demethylase 5C in 10 µg of human hippocampus tissue lysate.
Immunohistochemistry Analysis: A 1:500 dilution from a representative lot detected Lysine-specific demethylase 5C in human stomach tissue.
Immunohistochemistry Analysis: A 1:500 dilution from a representative lot detected Lysine-specific demethylase 5C in human stomach tissue.
Research Category
Epigenetics & Nuclear Function
Epigenetics & Nuclear Function
Research Sub Category
Histones
Histones
Quality
Evaluated by Western Blotting in human hippocampus tissue lysate.
Western Blotting Analysis: 2 µg/mL of this antibody detected Lysine-specific demethylase 5C in 10 µg of human hippocampus tissue lysate.
Western Blotting Analysis: 2 µg/mL of this antibody detected Lysine-specific demethylase 5C in 10 µg of human hippocampus tissue lysate.
Target description
~170 kDa observed. Uniprot describes four isoforms at 176 kDa (isoform 1), 175 kDa (isoform 2), 171 kDa (isoform 3), and 155 kDa (isoform 4). Uncharacterized band(s) may be observed in some cell lysates.
Physical form
Affinity purified
Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Storage and Stability
Stable for 1 year at 2-8°C from date of receipt.
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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