ABE490
Anti-NSD2 Antibody
from rabbit, purified by affinity chromatography
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Nuclear SET domain-containing protein 2, NSD2, Histone-lysine N-methyltransferase NSD2, Multiple myeloma SET domain-containing protein, MMSET, Protein trithorax-5 and Wolf-Hirschhorn syndrome candidate 1 protein, WHSC1
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biological source
rabbit
Quality Level
antibody form
affinity isolated antibody
antibody product type
primary antibodies
clone
polyclonal
purified by
affinity chromatography
species reactivity
human
technique(s)
immunohistochemistry: suitable
immunoprecipitation (IP): suitable
western blot: suitable
NCBI accession no.
UniProt accession no.
shipped in
wet ice
target post-translational modification
unmodified
Gene Information
human ... WHSC1(7468)
General description
Nuclear SET domain-containing protein 2 (NSD2) is also called Histone-lysine N-methyltransferase NSD2, Multiple myeloma SET domain-containing protein (MMSET), Protein trithorax-5 and Wolf-Hirschhorn syndrome candidate 1 protein (WHSC1). NSD2 is a histone methyltransferase that acts on histone H3 ′Lys-27′ (H3K27me). Isoform RE-IIBP is thought to be a transcription regulator that uses HADC recruitment to binding DNA and suppress IL5 transcription. Chromosomal aberrations to WHSC1 are linked to multiple myeloma tumors.
Specificity
This antibody is expected to cross-react with isoforms 1 (152 kDa), 3 (71 kDa), and 4 (66 kDa, REII-BP; IL-5 promoter REII-region-binding protein) based upon immunogen design.
Immunogen
KLH-conjugated linear peptide corresponding to a region near the C-terminus of human NSD2.
Application
Detect NSD using this rabbit polyclonal antibody, Anti-NSD2 Antibody validated for use in western blotting, IHC & IP.
Immunohistochemistry Analysis: A 1:500 dilution from a representative lot detected NSD2 in human cerebellum and thalamus tissues.
Immunohistochemistry Analysis: A 1:50 dilution from a representative lot detected NSD2 in human kidney tissues.
Immunoprecipitation Analysis: 5 µg of this antibody immunoprecipitated NSD2 from 500 µg of HCT-116 cell lysate.
Immunohistochemistry Analysis: A 1:50 dilution from a representative lot detected NSD2 in human kidney tissues.
Immunoprecipitation Analysis: 5 µg of this antibody immunoprecipitated NSD2 from 500 µg of HCT-116 cell lysate.
Research Category
Epigenetics & Nuclear Function
Epigenetics & Nuclear Function
Research Sub Category
Chromatin Biology
Chromatin Biology
Quality
Evaluated by Western Blotting in HeLa cell lysate.
Western Blotting Analysis: 2 µg/mL of this antibody detected NSD2 in 10 µg of HeLa cell lysate.
Western Blotting Analysis: 2 µg/mL of this antibody detected NSD2 in 10 µg of HeLa cell lysate.
Target description
~150 kDa observed. Uniprot describes 7 different isoforms ranging from 30 kDa to 152 kDa
Physical form
Affinity purified
Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Storage and Stability
Stable for 1 year at 2-8°C from date of receipt.
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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