All Photos(1)
Synonym(s):
Cyclic AMP-dependent transcription factor ATF-4, cAMP-dependent transcription factor ATF-4, Activating transcription factor 4, Cyclic AMP-responsive element-binding protein 2, CREB-2, cAMP-responsive element-binding protein 2
DNA-binding protein
DNA-binding protein
UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
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biological source
rabbit
Quality Level
antibody form
serum
antibody product type
primary antibodies
clone
polyclonal
species reactivity
human
technique(s)
ChIP: suitable
western blot: suitable
isotype
IgG
NCBI accession no.
UniProt accession no.
shipped in
wet ice
target post-translational modification
unmodified
Gene Information
human ... ATF4(468)
General description
Cyclic AMP-dependent transcription factor ATF-4 (ATF4) is a basic leucine-zipper (bZip) transcription factor, which regulates amino acid metabolism, DNA damage repair, chromatin remodeling, and apoptosis in response to cellular and ER stress. ATF4 works with various proteins, such as C/EBP homology protein (CHOP), aspargine synthetase (ASNS), and cAMP response element (CRE) among others to mediate cellular stress. ATF4 also regulates glucose homeostasis by suppressing beta-cell proliferation and insulin production. Furthermore, ATF4 targets the histone demethylase JMJD3 to alter chromatin structure and enhance gene transcription in response to amino acid deprivation.
Immunogen
Recombinant protein corresponding to human ATF4.
Application
Anti-ATF4 is an antibody targeting the ATF4 protein, validated for use in WB & ChIP.
Chromatin Immunoprecipitation Analysis: A representative lot from an independent laboratory detected ATF4 in HEPG2 cell lysates (Su, N. and Kilberg, M. S., et al. (2008). J Biol Chem. 283(50):351016-35117.; Fu, L., et al. (2011). J Biol Chem. 286(42):36724-36738.).
Chromatin Immunoprecipitation Analysis: A representative lot from an independent laboratory detected ATF4 in HEPG2 and MEF cell lysates (Shan, J., et al. (2012). J Biol Chem. 287(43):36393-36403.).
Chromatin Immunoprecipitation Analysis: A representative lot from an independent laboratory detected ATF4 in HEPG2 and MEF cell lysates (Shan, J., et al. (2012). J Biol Chem. 287(43):36393-36403.).
Research Category
Epigenetics & Nuclear Function
Epigenetics & Nuclear Function
Research Sub Category
Chromatin Biology
Chromatin Biology
Quality
Evaluated by Western Blot in untreated and HisOH treated HepG2 cell lysate.
Western Blot Analysis: A 1:2,000 dilution from a representative lot detected ATF4 in 10 µg of HisOH treated HepG2 cell lysate (HisOH was used to induce cellular stress mediated eIF2a phosphorylation, allowing for subsequent detection of ATF4. For enhanced performance, end-users may try higher dilutions of this antibody. For better results, 1% SDS was used during the secondary antibody incubation step.)
Western Blot Analysis: A 1:2,000 dilution from a representative lot detected ATF4 in 10 µg of HisOH treated HepG2 cell lysate (HisOH was used to induce cellular stress mediated eIF2a phosphorylation, allowing for subsequent detection of ATF4. For enhanced performance, end-users may try higher dilutions of this antibody. For better results, 1% SDS was used during the secondary antibody incubation step.)
Target description
~48 kDa observed. The calculated molecular weight is 39 kDa; however, this protein may be observed at ~48 kDa in human tissue lysates and ~45 kDa in mouse cell lysates due to post-translational modification. Uncharacterized bands may be observed at ~57 kDa, ~59 kDa, ~75 kDa, and ~100 kDa in some cell lysates.
Physical form
Rabbit polyclonal serum with 0.05% sodium azide.
Unpurified
Storage and Stability
Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Analysis Note
Control
HisOH treated HepG2 cell lysate
HisOH treated HepG2 cell lysate
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Certificates of Analysis (COA)
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