Anti-NFκB p65 Antibody, CT

The transcription factor NFκB (Nuclear Factor κB) is involved in the expression and regulation of a number of important cellular and physiological processes such as growth, development, apoptosis, immune and inflammatory response, and activation of various viral promoters including human immunodeficiency virus long terminal repeats. NFκB represents a group of structurally related and evolutionarily conserved proteins related to the proto-oncogene c-Rel with five members in mammals that include Rel (cRel), RelA (p65), RelB, NFκB1 (p50 and its precursor p105), and NFκB2 (p52 and its precursor p100). NFκB/Rel proteins exist as homo- or heterodimers to form transcriptionally competent or repressive complexes. Although most NFκB dimers are activators of transcription, the p50/50 and p52/52 homodimers can repress the transcription of their target genes. The p50/p65 heterodimer of NFκB is the most abundant in cells.

This antibody recognizes NFκB at the C-terminus.

Epitope: C-terminus

KLH-conjugated linear peptide corresponding to the C-terminus of human NFκB.

Research Category
Epigenetics & Nuclear Function

Research Sub Category
Transcription Factors

Use Anti-NFκB p65 Antibody, CT (Rabbit Polyclonal Antibody) validated in WB, IP to detect NFκB p65 also known as transcription factor p65.

Western Blot (SNAP i.d.) Analysis: 5 µg/mL from a representative lot detected NFκB on 10 µg of A431 cell lysate.

Immunoprecipitation Analysis: 10 µg from a representative lot immunoprecipitated NFκB from A431 RIPA lysate.

Evaluated by Western Blot in A431 cell lysate.

Western Blot Analysis: 0.5 µg/mL of this antibody detected NFκB in 10 µg of A431 cell lysate.

~65 kDa observed.
Four isoforms produced by alternative splicing have been described: Isoform 1 (p65) at 60.2 kDa, Isoform 2 (p65 delta 2) at 58.8 kDa, Isoform 3 (p65 delta) at 59.1 kDa, and Isoform 4 at 59.9 kDa

Replaces: 06-418

Format: Purified

Protein A purified

Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.05% sodium azide.

Stable for 1 year at 2-8°C from date of receipt.

Control
A431 cell lysate

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

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