ABE217
Anti-CUX1 Antibody, a.a. 861
from rabbit, purified by affinity chromatography
Synonym(s):
Homeobox protein cut-like 1, CCAAT displacement protein, CDP, Homeobox protein cux-1
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About This Item
UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
biological source
rabbit
Quality Level
antibody form
affinity isolated antibody
antibody product type
primary antibodies
clone
polyclonal
purified by
affinity chromatography
species reactivity
human
technique(s)
immunoprecipitation (IP): suitable
western blot: suitable
NCBI accession no.
UniProt accession no.
shipped in
wet ice
target post-translational modification
unmodified
Gene Information
human ... CUX1(1523)
General description
Homeobox protein cut-like 1 (CUX1) is also known as CCAAT displacement protein (CDP) and Homeobox protein cux-1. CUX1 is thought to repress expression of developmentally regulated genes involved in mammalian development, likely through the prevention of CCAAT factor binding at promoters. CUX1 also represses T-cell receptor (TCR) beta enhancer function by binding to matrix attachment region beta (MARbeta), and is a component of the nf-munr repressor, which binds to matrix attachment regions (MARs) of the immunoglobulin heavy chain enhancer.
Specificity
Other homologies: Mouse (95% sequence homology).
This antibody recognizes the region of amino acids beginning at 861 of CUX1. This antibody is expected to cross react with all known isoforms of CUX1.
Immunogen
Epitope: Region of amino acids beginning at 861
GST-tagged recombinant protein corresponding to a region of amino acids beginning at 861 of human CUX1.
Application
Detect Cux1 using this rabbit polyclonal antibody, Anti-CUX1 Antibody, a.a. 861 validated for use in western blotting & IP.
Immunoprecipitation Analysis: 1.25 µg from a representative lot immunoprecipitated CUX1 in 500 µg of HeLa nuclear extract.
Alexa Fluor™ is a registered trademark of Life Technologies.
Alexa Fluor™ is a registered trademark of Life Technologies.
Research Category
Epigenetics & Nuclear Function
Epigenetics & Nuclear Function
Research Sub Category
Transcription Factors
Transcription Factors
Quality
Evaluated by Western Blotting in HeLa nuclear extract.
Western Blotting Analysis: 0.25 µg/mL from a representative lot detected CUX1 in 10 µg of HeLa nuclear extract.
Western Blotting Analysis: 0.25 µg/mL from a representative lot detected CUX1 in 10 µg of HeLa nuclear extract.
Target description
~200 kDa and ~110 kDa observed. Uniprot describes 11 different isoforms. A full length protein has been described at 200 kDa and is proteolytically cleaved into the p150, p110, p90, and p80 isoforms. Additionally, the p75 isoform is the result of alternative splicing (Sansregret, L, et al. (2008). Gene. 412(1-2):84-94.). Uncharacterized band(s) may be observed in some cell lysates.
Physical form
Affinity purified
Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Storage and Stability
Stable for 1 year at 2-8°C from date of receipt.
Analysis Note
Control
HeLa nuclear extract
HeLa nuclear extract
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Legal Information
ALEXA FLUOR is a trademark of Life Technologies
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class Code
12 - Non Combustible Liquids
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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