All Photos(1)
Synonym(s):
PAXIP1-associated glutamate-rich protein 1, Glutamate-rich co-activator interacting with SRC11, GAS1, PAXIP1-associated protein 1, PTIP-associated protein 1
UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
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biological source
rabbit
Quality Level
antibody form
affinity isolated antibody
antibody product type
primary antibodies
clone
polyclonal
species reactivity
mouse, human
packaging
antibody small pack of 25 μg
technique(s)
ChIP: suitable
immunocytochemistry: suitable
western blot: suitable
isotype
IgG
NCBI accession no.
UniProt accession no.
shipped in
ambient
target post-translational modification
unmodified
Gene Information
human ... PAGR1(79447)
Related Categories
General description
PAXIP1-associated glutamate-rich protein 1 (UniProt: Q9BTK6; also known as Glutamate-rich co-activator interacting with SRC1, GAS, PAXIP1-associated protein 1, PTIP-associated protein 1) is encoded by the PAGR1 (also known as C16orf53, PA1) gene (Gene ID: 79447) in human. It associates with the histone methyltransferase MLL3/4 complex, which suggests that it plays a role in epigenetic and transcriptional activation. However, in association with PAXIP1/PTIP is proposed to function at least in part independently of the MLL3/4 complex. It is proposed to be recruited by PAXIP1 to sites of DNA damage where the PAGR1:PAXIP1 complex is required for cell survival in response to DNA damage independently of the MLL3/4 complex. During immunoglobulin class switching in activated B-cells, it is involved in transcription regulation of downstream switch regions at the immunoglobulin heavy-chain (Igh) locus independently of the MLL3/4 complex. PAGR1 protein is reported to act as a transcriptional cofactor for estrogen receptor. It interacts with GR (NR3C1) and inhibits the GR transactivation.
Specificity
This polyclonal antibody detects PAXIP1-associated glutamate-rich protein 1 in human and mouse.
Immunogen
His-tagged full-length human recombinant PAXIP1-associated protein 1.
Application
Anti-PAGR1 (PA1), Cat. No. ABE1863, is a highly specific rabbit polyclonal antibody that targets PAXIP1-associated glutamate-rich protein 1 and has been tested in Chromatin Immunoprecipitation (ChIP), Immunocytochemistry, and Western Blotting.
Western Blotting Analysis: A representative lot detected PAGR1 (PA1) in Western Blotting applications (Lee, J.E., et. al. (2013). Elife. 2:e01503; Gong, Z., et. al. (2009). J Biol Chem. 284(11):7284-93).
Chromatin Immunoprecipitation Analysis: A representative lot detected PAGR1 (PA1) in MEF′s (Cho, Y.W., et. al. (2009). Cell Metab. 10(1):27-39).
Immunocytochemistry Analysis: A representative lot detected PAGR1 (PA1) in U2OS & HeLa cells (Cho, Y.W., et. al. (2009). Cell Metab. 10(1):27-39).
Chromatin Immunoprecipitation Analysis: A representative lot detected PAGR1 (PA1) in MEF′s (Cho, Y.W., et. al. (2009). Cell Metab. 10(1):27-39).
Immunocytochemistry Analysis: A representative lot detected PAGR1 (PA1) in U2OS & HeLa cells (Cho, Y.W., et. al. (2009). Cell Metab. 10(1):27-39).
Quality
Evaluated by Western Blotting in HEK293 cell nuclear extract.
Western Blotting Analysis: 0.5 µg/mL of this antibody detected PAGR1 (PA1) in 10 µg of HEK293 cell nuclear extract.
Western Blotting Analysis: 0.5 µg/mL of this antibody detected PAGR1 (PA1) in 10 µg of HEK293 cell nuclear extract.
Target description
~42 kDa observed; 27.72 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
Physical form
Format: Purified
Other Notes
Concentration: Please refer to lot specific datasheet.
WGK
WGK 1
Certificates of Analysis (COA)
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