Anti-CDK12 Antibody

Cyclin-dependent kinase 12 (EC 2.7.11.22; EC 2.7.11.23; UniProt Q9NYV4; also known as CDC2-related protein kinase 7, Cdc2-related kinase, arginine/serine-rich, Cell division cycle 2-related protein kinase 7, Cell division protein kinase 12, CrkRS, hCDK12) is encoded by the CDK12 (also known as CRK7, CRKRS, KIAA0904) gene (Gene ID 51755) in human. CDK12 belongs to the CMGC Ser/Thr protein kinase subfamily of cyclin-dependent kinases (CDKs). Originally identified and characterized as an RNA Polymerase II C-terminal domain (CTD) kinase, CDK12 is involved in the 3′ end processing of the MYC and c-FOS mRNA transcripts. In addtion, CDK12/CyclinK complex is implicated in the maintenance of genomic stability through the regulation of DNA damage response genes and as a determinant of PARP1/2 inhibitor sensitivity in cancer treatment. Human CDK12 is uncharacteristically large with a molecular weight of ∼160 kDa (most CDKs are about 50 kDa) and contains large N- and C-terminal extensions flanking the kinase domain (a.a. 727-1020). These extensions contain multiple stretches of low complexity sequence and RS domains characteristic of splicing factors and regulators believed to mediate protein-protein interactions. As expected of RS domain-containing proteins, CDK12 is found in nuclear speckles representing storage sites for splicing factors.

Immunogen sequence is present in all spliced isoforms of human, mouse, and rat CDK12 reported by UniProt (Q9NYV4, Q14AX6, and Q3MJK5).

Linear peptide corresponding to an N-terminal sequence of human CDK12.

Anti-CDK12 Antibody, Cat. No. ABE1861, is a highly specific rabbit polyclonal antibody that targets CDK12 and has been tested in Immunoprecipitation and Western Blotting.

Western Blotting Analysis: 0.2 µg/mL from a representative lot detected CDK12 in 45 µg of HeLa nuclear extract (Courtesy of Dr. Arno Greenleaf, Duke University, Durham, NC, U.S.A.).
Western Blotting Analysis: A representative lot detected baculovirus expressed human CDK12 in lysates from infected Sf9 cells (Bartkowiak, B., and Greenleaf, A.L. (2015). J. Biol. Chem. 290(3):1786-1795).
Western Blotting Analysis: A representative lot detected CDK12 in HeLa nuclear extract, as well as RNAi-mediated CDK12 downregulation in HEK293T/17 cells (Schwartz, J.C., et al. (2012). Genes Dev. 26(24):2690-2695).
Immunoprecipitation Analysis: Representative lots immunoprecipitated CDK12 from HeLa nuclear extract (Bartkowiak, B., and Greenleaf, A.L. (2015). J. Biol. Chem. 290(3):1786-1795; Schwartz, J.C., et al. (2012). Genes Dev. 26(24):2690-2695).

Evaluated by Western Blotting in HeLa nuclear extract.

Western Blotting Analysis: 2.0 µg/mL of this antibody detected CDK12 in 10 µg of HeLa nuclear extract.

~200 kDa observed. Target band size appears larger than the calculated molecular weights of 164.2/163.2/141.4 kDa (human isoform 1/2/3), 163.7/139.9/162.8 kDa (rat isoform 1/2/3), and 163.8/140.0 kDa (rat isoform 1/2), likely due to posttranslational modifications such as phosphorylation. Uncharacterized band(s) may appear in some lysates.

Concentration: Please refer to lot specific datasheet.