ABD130
Anti-PRDM16 Antibody
from rabbit, purified by affinity chromatography
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Synonym(s):
PR domain containing 16, MEL1, PFM13, KIAA1675, MEL1, MDS1/EVI1-like gene 1, Transcription factor MEL1, PR domain zinc finger protein 16
UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Recommended Products
biological source
rabbit
Quality Level
antibody form
affinity isolated antibody
antibody product type
primary antibodies
clone
polyclonal
purified by
affinity chromatography
species reactivity
rat, human
technique(s)
immunofluorescence: suitable
immunohistochemistry: suitable
western blot: suitable
UniProt accession no.
shipped in
wet ice
target post-translational modification
unmodified
Gene Information
human ... PRDM16(63976)
General description
PR domain zinc finger protein 16 (PRDM16) is also called Transcription factor MEL1 (MDS1/EVI1-like gene 1). PRDM16 is a zinc finger transcription factor and contains an N-terminal PR domain. PRDM16 is expressed in the uterus and kidney. PRDM16 regulates transcription through DNA binding and plays a role in the differentiation of myoblastic precursors into brown adipose tissue (BAT), and represses TGF-beta signalling. Translocations in PRDM16 occur in subsets of myelodysplastic syndrome (MDS) and acute myeloid leukemia (AML) cases.
Specificity
This antibody can recognize all four isoforms of PRDM16.
Immunogen
KLH-conjugated linear peptide corresponding to human PRDM16.
Application
Detect PRDM16 using this rabbit polyclonal antibody, Anti-PRDM16 Antibody validated for use in western blotting, IHC & Immunofluorescence.
Immunohistochemistry Analysis: 2.5 µg/mL from a representative lot detected PRDM16 in rat brain tissue.
Immunofluorescence Analysis: 20 μg/mL from a representative lot detected PRDM16 in human brain cells.
Immunofluorescence Analysis: 20 μg/mL from a representative lot detected PRDM16 in human brain cells.
Research Category
Stem Cell Research
Stem Cell Research
Research Sub Category
Pluripotent & Early Differentiation
Pluripotent & Early Differentiation
Quality
Evaluated by Western Blotting in rat brain tissue lysate.
Western Blotting Analysis: 2 µg/mL of this antibody detected PRDM16 in 15 µg of rat brain tissue lysate.
Western Blotting Analysis: 2 µg/mL of this antibody detected PRDM16 in 15 µg of rat brain tissue lysate.
Target description
~100 kDa, ~135 kDa and ~150 kDa observed. Uncharacterized bands may appear in some lysate(s). Uniprot describes 4 isoforms between ~120 kDa and ~140 kDa
Physical form
Antigen Affinity Purified
Purified rabbit polyclonal in buffer containing PBS with up to 0.1% sodium azide.
Storage and Stability
Stable for 1 year at 2-8°C from date of receipt.
Analysis Note
Control
Rat brain tissue lysate
Rat brain tissue lysate
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
WGK
WGK 2
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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S Wang et al.
International journal of obesity (2005), 39(6), 967-976 (2015-03-13)
Development of brown-like/beige adipocytes in white adipose tissue (WAT) helps to reduce obesity. Thus we investigated the effects of resveratrol, a dietary polyphenol capable of preventing obesity and related complications in humans and animal models, on brown-like adipocyte formation in
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