All Photos(4)
Synonym(s):
NADPH oxidase 4, Kidney oxidase-1, KOX-1, Kidney superoxide-producing NADPH oxidase, Renal NAD(P)H-oxidase
UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
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biological source
rabbit
Quality Level
antibody form
purified antibody
antibody product type
primary antibodies
clone
polyclonal
species reactivity
human, mouse
species reactivity (predicted by homology)
rat (based on 100% sequence homology)
technique(s)
immunocytochemistry: suitable
immunohistochemistry: suitable
NCBI accession no.
UniProt accession no.
shipped in
wet ice
target post-translational modification
unmodified
Gene Information
human ... NOX4(50507)
General description
NOX4 (NADPH oxidase 4) is a phagocyte-type oxidase, similar to that responsible for the production of large amounts of reactive oxygen species (ROS) in neutrophil granulocytes with resultant antimicrobial activity and has been postulated to function in the kidney as an oxygen sensor that regulates the synthesis of erythropoietin in the renal cortex.
Immunogen
Linear peptide corresponding to the C-terminus of human NOX4.
Application
Anti-NOX4 Antibody is a highly specific rabbit polyclonal antibody, that targets NADPH Oxidase (phox) & has been tested in IHC & ICC.
Immunohistochemistry Analysis: A 1:500 and a 1:50 dilution from a representative lot detected NOX4 in human kidney and skeletal muscle tissues, respectively.
Immunohistochemistry Analysis:: A representative lot detected NOX4 in mouse liver tissue (I. Fabregat, IDIBELL, Barcelona, Spain).
Immunocytochemistry Analysis: A representative lot detected NOX4 in Hep3B cells (Esther Bertran, IDIBELL, Barcelona, Spain).
Immunohistochemistry Analysis: A representative lot detected NOX4 in mouse liver tissues expressing wildtype Mdr2/p19, or knockout Mdr2 ARF or knockout Stat3/Mdr2 (Sancho, P. et al. (2012). PLoS One. 7(9):e45285.).
Immunohistochemistry Analysis:: A representative lot detected NOX4 in mouse liver tissue (I. Fabregat, IDIBELL, Barcelona, Spain).
Immunocytochemistry Analysis: A representative lot detected NOX4 in Hep3B cells (Esther Bertran, IDIBELL, Barcelona, Spain).
Immunohistochemistry Analysis: A representative lot detected NOX4 in mouse liver tissues expressing wildtype Mdr2/p19, or knockout Mdr2 ARF or knockout Stat3/Mdr2 (Sancho, P. et al. (2012). PLoS One. 7(9):e45285.).
Research Category
Apoptosis & Cancer
Apoptosis & Cancer
Research Sub Category
Apoptosis - Additional
Apoptosis - Additional
Quality
Evaluated by Immunohistochemistry in human kidney tissue.
Immunohistochemistry Analysis: A 1:50 dilution of this antibody detected NOX4 in human kidney tissue.
Immunohistochemistry Analysis: A 1:50 dilution of this antibody detected NOX4 in human kidney tissue.
Target description
67 kDa calculated
Physical form
Format: Purified
Protein A purified
Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Storage and Stability
Stable for 1 year at 2-8°C from date of receipt.
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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Joaquim Moreno-Càceres et al.
Cell death & disease, 8(10), e3098-e3098 (2017-10-13)
Hepatocellular carcinoma (HCC) is a heterogeneous tumour associated with poor prognostic outcome. Caveolin-1 (CAV1), a membrane protein involved in the formation of caveolae, is frequently overexpressed in HCC. Transforming growth factor-beta (TGF-β) is a pleiotropic cytokine having a dual role
Yung-Chih Chen et al.
Journal of the American Heart Association, 11(1), e022761-e022761 (2022-01-01)
Background Diabetes is known to accelerate atherosclerosis and increase plaque instability. However, there has been a lack of suitable animal models to study the effect of diabetes on plaque instability. We hypothesized that the tandem stenosis mouse model, which reflects
Becky A Diebold et al.
Methods in molecular biology (Clifton, N.J.), 1982, 191-229 (2019-06-07)
The identification of NADPH oxidase (NOX) isoforms in tissues is essential for interpreting experiments and for next step decisions regarding cell lines, animal models, and targeted drug design. Two basic methods, immunoblotting and reverse transcriptase-quantitative polymerase chain reaction (RT-qPCR), are
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