Anti-Trop-2 Antibody

The protein known as Trop-2, or also known as cell surface glycoprotein Trop-2, Membrane component chromosome 1 surface marker 1 or Pancreatic carcinoma marker protein GA733-1 and encoded by the gene TACSTD2/GA733-1/M1S1/TROP2, is a protein that may function as a growth factor receptor and is expressed primarily in the placenta, lung and epithelial tissues but also found expressed in pancreatic cancer cell lines. Interestingly, this protein′s gene is intronless, a rare event in eukaryotic genes, and was first identified as a carcinoma antigen by the monoclonal GA733. It is a plasma membrane bound protein that transduces an intracellular calcium signal and appears to act as a receptor for as yet unidentified growth factors. Interestingly too, mutations in this gene are associated with a rare cause of blindness with amyloid protein bodies (insoluble fibrous protein aggregates but not always of amyloid protein) beneath the cornea. Trop-2 has also recently been shown to be important in regulating fibroblast motility and lung cell proliferation and does so by regulating the ERK kinase pathway as well as several critical components of the cell migration apparatus.

Epitope: Intracellular domain

Linear peptide corresponding to the intracellular domain of human Trop-2.

Anti-Trop-2 Antibody detects level of Trop-2 & has been published & validated for use in Trop-2.

Research Category
Apoptosis & Cancer

Research Sub Category
Apoptosis - Additional

Western Blotting Analysis: 1.0 µg/mL from a representative lot detected Trop-2 in 10 µg of HT1376, SiHa, A549, TERA-2, T47D, OVCAR 3, Colo-587, PANC-1, JEG-3, HCC1143, DU145, NT2/D1 , SK-OV-3, LNCap cell lysate.
Immunohistochemistry Analysis: A representative lot detected Trop-2 in in human prostate cancer tissues (Stoyanova, T., et al. (2012). Genes Dev. 26:2271-2285).

Evaluated by Western Blotting in Tera-2 cell lysate.

Western Blotting Analysis: 1.0 µg/mL of this antibody detected Trop-2 in 10 µg of Tera-2 cell lysate.

~ 36 kDa observed. Uncharacterized band(s) may appear in some lysates.

Format: Purified

Protein G Purified

Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Stable for 1 year at 2-8°C from date of receipt.

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.