ABC347
Anti-Fn14 Antibody
from rabbit, purified by affinity chromatography
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Fn14 Antibody, Fibroblast growth factor-inducible 14, TNFRSF12, TWEAK-R, FGF-inducible 14
Recommended Products
biological source
rabbit
Quality Level
antibody form
affinity isolated antibody
antibody product type
primary antibodies
clone
polyclonal
purified by
affinity chromatography
species reactivity
human
technique(s)
immunofluorescence: suitable
immunohistochemistry: suitable
western blot: suitable
UniProt accession no.
shipped in
wet ice
target post-translational modification
unmodified
Gene Information
human ... TNFRSF12A(51330)
General description
Fibroblast growth factor 14 (FN14) is a member of the TNF receptor superfamily. FN14 is a receptor for TNFSF12/TWEAK and promotes angiogenesis and the proliferation of endothelial cells. Experiments have shown that FN14 binds to the TNFR-associated factors TRAF1, TRAF2, TRAF3, and TRAF5, suggesting that the signaling pathway induced by TWEAK may be mediated by one or more of these adaptor molecules. Additionally, FN14 is a weak inducer of apoptosis in some cell types and may modulate cellular adhesion to matrix proteins. FN14 is suggested to induce liver regeneration and is highly expressed in hepatocellular carcinomas. Furthermore, TWEAK induces liver progenitor cell proliferation, suggesting FN14 may play a role in hepatocyte growth control and liver neoplasia. FN14 is highly expressed in heart, placenta and kidney. Intermediate expression of FN14 has been recorded in lung, skeletal muscle and pancreas.
Immunogen
KLH-conjugated Fc-tagged protein corresponding to human Fn14.
Application
Anti-Fn14 Antibody is a highly specific rabbit polyclonal antibody, that targets Fibroblast growth factor 14 & has been tested in western blotting, IHC & Immunofluorescence.
Immunohistochemistry Analysis: 2.5 µg/mL from a representative lot detected Fn14 in human liver tissue lysate.
Immunofluorescence Analysis: 20 µg/mL from a representative lot detected Fn14 in human liver tissue.
Immunofluorescence Analysis: 20 µg/mL from a representative lot detected Fn14 in human liver tissue.
Research Category
Apoptosis & Cancer
Apoptosis & Cancer
Research Sub Category
Apoptosis - Additional
Apoptosis - Additional
Quality
Evaluated by Western Blotting in HepG2 cell lysate.
Western Blotting Analysis: 4 µg/mL of this antibody detected Fn14 in 15 µg of HepG2 cell lysate.
Western Blotting Analysis: 4 µg/mL of this antibody detected Fn14 in 15 µg of HepG2 cell lysate.
Target description
~34 kDa observed. Uncharacterized bands may appear in some lysate(s). Uniprot describes 2 isoforms at ~10 kDa and ~14 kDa
Physical form
Antigen Affinity Purified
Purified rabbit polyclonal in buffer containing PBS with up to 0.1% sodium azide.
Storage and Stability
Stable for 1 year at 2-8°C from date of receipt.
Analysis Note
Control
HepG2 cell lysate
HepG2 cell lysate
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
WGK
WGK 2
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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