ABC299
Anti-FFAR2 Antibody/GPR43
from rabbit, purified by affinity chromatography
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All Photos(3)
Synonym(s):
Free fatty acid receptor 2, G-protein coupled receptor 43, FFAR2/GPR43
UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Recommended Products
biological source
rabbit
Quality Level
antibody form
affinity isolated antibody
antibody product type
primary antibodies
clone
polyclonal
purified by
affinity chromatography
species reactivity
rat
species reactivity (predicted by homology)
human (based on 100% sequence homology), mouse
technique(s)
immunohistochemistry: suitable
western blot: suitable
NCBI accession no.
UniProt accession no.
shipped in
wet ice
target post-translational modification
unmodified
Gene Information
human ... FFAR2(2867)
General description
Free fatty acid receptor 2 (FFAR2), also known as G-protein coupled receptor 43 (GPR43), functions as the cell membrane receptor for short chain fatty acids that ultimately stimulates G protein coupled, inhibitory (Gi) signals via the adenylate cyclase pathway. FFAR2/GPR43 is found expressed in leukocytes in the lamina propria of the small intestine mostly but some expression can be also be found in splenocytes, adipose tissue, and enteroendocrine cells of the gut. Interesting, FFAR2/GPR43 because it senses short chain fatty acids which are often key metabolites of gut microbiota, it may serve as a biological sensor to regulate the activity of regulatory T cells and the immune response in diseases such as colitis.
Immunogen
Epitope: Cytoplasmic domain
GST-tagged recombinant protein corresponding to the cytoplasmic domain of human FFAR2/GPR43.
Application
Research Category
Apoptosis & Cancer
Apoptosis & Cancer
Research Sub Category
Apoptosis - Additional
Apoptosis - Additional
This Anti-FFAR2 Antibody/GPR43 is validated for use in Western Blotting and Immunohistochemistry for the detection of FFAR2/GPR43.
Western Blotting Analysis: 1.0 µg/mL from a representative lot detected FFAR2/GPR43 in 10 µg of RAW264.7 cell lysate.
Immunohistochemistry Analysis: A 1:50 dilution from a representative lot detected FFAR2/GPR43 in mouse kidney and mouse liver tissue.
Immunohistochemistry Analysis: A 1:50 dilution from a representative lot detected FFAR2/GPR43 in mouse kidney and mouse liver tissue.
Quality
Evaluated by Western Blotting in CHEM-1 cell lysate.
Western Blotting Analysis: 1.0 µg/mL of this antibody detected FFAR2/GPR43 in 10 µg of CHEM-1 cell lysate.
Western Blotting Analysis: 1.0 µg/mL of this antibody detected FFAR2/GPR43 in 10 µg of CHEM-1 cell lysate.
Target description
~47 kDa observed. The calculated molecular weight of this protein is 37 kDa, but can be observed at ~47 kDa due to high glycosylation. Uncharacterized band(s) may appear in some lysates.
Physical form
Affinity purified
Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Storage and Stability
Stable for 1 year at 2-8°C from date of receipt.
Other Notes
Concentration: Please refer to lot specific datasheet.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
WGK
WGK 1
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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Hideaki Fujiwara et al.
Nature communications, 9(1), 3674-3674 (2018-09-12)
Microbiome-derived metabolites influence intestinal homeostasis and regulate graft-versus-host disease (GVHD), but the molecular mechanisms remain unknown. Here we show the metabolite sensor G-protein-coupled receptor 43 (GPR43) is important for attenuation of gastrointestinal GVHD in multiple clinically relevant murine models. GPR43
Yung-Mei Chao et al.
Nutrients, 14(20) (2022-10-28)
The role of short-chain fatty acids (SCFAs) in the brain on the developmental programming of hypertension is poorly understood. The present study explored dysregulated tissue levels of SCFAs and expression of SCFA-sensing receptors in the hypothalamic paraventricular nucleus (PVN), a
Qian-Jiang Luo et al.
Gastroenterology report, 9(2), 154-165 (2021-05-25)
Butyrate acts as a regulator in multiple inflammatory organ injuries. However, the role of butyrate in acute liver injury has not yet been fully explored. In the present study, we aimed to investigate the association between butyrate and lipopolysaccharide (LPS)-induced
Ting-Bei Bo et al.
The ISME journal, 13(12), 3037-3053 (2019-08-29)
Gut microbiota play a critical role in orchestrating metabolic homeostasis of the host. However, the crosstalk between host and microbial symbionts in small mammals are rarely illustrated. We used male Brandt's voles (Lasiopodomys brandtii) to test the hypothesis that gut
Zahra Nouri et al.
NPJ biofilms and microbiomes, 8(1), 16-16 (2022-04-06)
Regulating sodium and water balances is crucial for survival of small, desert mammals. Studies demonstrate that the gut microbiota and their metabolites are involved in host energy homeostasis, but little is known on the interactions among salt loading, gut microbiota
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