Anti-Sodium-Iodide Symporter Antibody, C-terminal 1

Sodium/iodide cotransporter (UniProt: Q92911; also known as Na(+)/I(-) cotransporter, Sodium-iodide symporter, Na(+)/I(-) symporter) is encoded by the SLC5A5 (also known as NIS) gene (Gene ID: 6528) in human. Sodium/iodide cotransporter is a multi-pass membrane protein that mediates iodide uptake in thyroid gland. It is expressed primarily in thyroid tissue, but is present in lower levels in mammary gland and ovary. It expression is significantly reduced in tumors. The presence of symporter in mammary glands leads to secretion of iodine in milk that is required for thyroid function in neonatal animals. NIS gene and protein expression is stimulated by thyroid-stimulating hormone. Both human and rat NIS contain N-glycosylation sites, however, glycosylation is not required for its full activity. Abnormalities in the expression of NIS gene lead to thyroid disease and can result in congenital hypothyroidism. Autoantibodies to NIS adversely affect iodide transport.

This rabbit polyclonal antibody detects Sodium-iodide symporter in human. It targets an epitope within 16 amino acids from the C-terminal region. For Western blotting application, please do not boil the sample to avoid any aggregation. When transfering protein to nitrocellulose membrane use of 0.05% (w/v) lithium dodecyl sufate is highly recommended in the transfer buffer.

Epitope: cytoplasmic domain

KLH-conjugated linear peptide corresponding to 16 amino acids from the C-terminal region of human Sodium-iodide symporter.

Anti-Sodium-Iodide Symporter, C-terminal 1, Cat. No. ABC1455, is a rabbit polyclonal antibody that detects Sodium/iodide cotransporter and has been tested for use in Immunohistochemistry (Paraffin) and Western Blotting.

Immunohistochemistry Analysis: A 1:50-250 dilution from a representative lot detected Sodium-Iodide Symporter in human thyroid and human salivary gland tissue sections.

Western Blotting Analysis: A representative lot detected Sodium-Iodide Symporter in Western Blotting applications (Nicola, J.P., et. al. (2015). J Clin Endocrinol Metab. 100(10):E1353-61).

Western Blotting Analysis: A 1:5,000 dilution from a representative lot detected Sodium-Iodide Symporter in MDCK cells transduced with hNIS (Courtesy of Dr Nancy Carrasco & Dr. Andrea Reyna-Neyra at Yale University).

Research Category
Apoptosis & Cancer

Evaluated by Immunohistochemistry in human stomach tissue sections.

Immunohistochemistry Analysis: A 1:50 dilution of this antibody detected Sodium-Iodide Symporter in human stomach tissue sections.

68.66 kDa calculated.

Affinity Purified

Format: Purified

Purified rabbit polyclonal antibody in buffer containing 76 mM Tris, 192 mM glycine, pH 7.4 with 0.05% sodium azide.

Stable for 1 year at 2-8°C from date of receipt.

Concentration: Please refer to lot specific datasheet.

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.