All Photos(4)
Synonym(s):
BCL2-associated X protein, Bcl-2-like protein 4, apoptosis regulator BAX
UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
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biological source
rabbit
Quality Level
antibody form
purified antibody
antibody product type
primary antibodies
clone
polyclonal
species reactivity
human
species reactivity (predicted by homology)
bovine (based on 100% sequence homology), feline (based on 100% sequence homology), canine (based on 100% sequence homology), chimpanzee (based on 100% sequence homology), rhesus monkey (based on 100% sequence homology)
technique(s)
immunocytochemistry: suitable
immunohistochemistry: suitable
immunoprecipitation (IP): suitable
western blot: suitable
NCBI accession no.
UniProt accession no.
shipped in
wet ice
target post-translational modification
unmodified
Gene Information
human ... BAX(581)
General description
BAX (BCL2-associated X protein) belongs to the BCL2 protein family. BCL2 family members form hetero- or homodimers and act as anti- or pro-apoptotic regulators that are involved in a wide variety of cellular activities. BAX protein forms a heterodimer with BCL2, and functions as an apoptotic activator. This protein is reported to interact with, and increase the opening of, the mitochondrial voltage-dependent anion channel (VDAC), which leads to the loss in membrane potential and the release of cytochrome c. The expression of this gene is regulated by the tumor suppressor P53 and has been shown to be involved in P53-mediated apoptosis. Multiple alternatively spliced transcript variants, which encode different isoforms, have been reported for this gene.
The previously assigned protein identifier Q07815 has been merged into Q07812. Full details can be found on the UniProt database.
Specificity
Rat (90%), Mouse (86%).
This antibody recognizes BAX.
Immunogen
KLH-conjugated linear peptide corresponding to human BAX at and around the N-terminus.
Application
Anti-Bax Antibody is an antibody against Bax (N-terminus) for use in WB, IP, IC, IH.
Immunoprecipitation Analysis: 10 µg from a previous lot immunoprecipitated BAX from 500 µg of HL60 cell lysate.
Immunohistochemistry Analysis: 1:300 dilution from a previous lot detected BAX in colorectal and ductal carcinoma tissue.
Immunocytochemistry Analysis: 1:500 dilution from a previous lot detected BAX in MCF7 cells.
Immunohistochemistry Analysis: 1:300 dilution from a previous lot detected BAX in colorectal and ductal carcinoma tissue.
Immunocytochemistry Analysis: 1:500 dilution from a previous lot detected BAX in MCF7 cells.
Quality
Evaluated by Western Blot in HL60 cell lysate.
Western Blot Analysis: 0.125 µg/ml of this antibody detected BAX on 10 µg of HL60 cell lysate.
Western Blot Analysis: 0.125 µg/ml of this antibody detected BAX on 10 µg of HL60 cell lysate.
Target description
~ 21 kDa
Linkage
Replaces: 06-499
Physical form
Format: Purified
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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Dan-Dan Sun et al.
Journal of dental sciences, 18(1), 310-321 (2023-01-17)
Periodontitis is a prevalent infectious inflammatory disease. Growing evidence has revealed important roles for circular RNAs (circRNAs) and circRNA sponge activity in periodontitis. Here, we elucidated the precise part of circ_0097010 in periodontitis pathogenesis. Human periodontal ligament cells (hPDLCs) were
Adeline Y Robin et al.
Structure (London, England : 1993), 26(10), 1346-1359 (2018-08-21)
BAX and BAK are essential mediators of intrinsic apoptosis that permeabilize the mitochondrial outer membrane. BAX activation requires its translocation from cytosol to mitochondria where conformational changes cause its oligomerization. To better understand the critical step of translocation, we examined
Teruyoshi Saito et al.
Oncology reports, 29(1), 335-342 (2012-11-06)
Hyaluronan (HA), a major component of the extracellular matrix (ECM), is synthesized by HA synthase (HAS) 1, HAS2 and HAS3 and is intricately involved
Bo Zhou et al.
Cell research, 28(12), 1171-1185 (2018-10-06)
Iron has been shown to trigger oxidative stress by elevating reactive oxygen species (ROS) and to participate in different modes of cell death, such as ferroptosis, apoptosis and necroptosis. However, whether iron-elevated ROS is also linked to pyroptosis has not
Sweta Iyer et al.
Nature communications, 7, 11734-11734 (2016-05-25)
During apoptosis, Bak and Bax are activated by BH3-only proteins binding to the α2-α5 hydrophobic groove; Bax is also activated via a rear pocket. Here we report that antibodies can directly activate Bak and mitochondrial Bax by binding to the
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