AB9878
Anti-MAGI-2 Antibody
Chemicon®, from rabbit
Synonym(s):
Membrane Associated Guanylate Kinase, WW and PDZ domain containing 2, S-SCAM, Synaptic Scaffolding Molecule
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About This Item
UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
biological source
rabbit
antibody form
purified antibody
antibody product type
primary antibodies
clone
polyclonal
species reactivity
mouse
manufacturer/tradename
Chemicon®
technique(s)
immunocytochemistry: suitable
immunohistochemistry: suitable
immunoprecipitation (IP): suitable
western blot: suitable
NCBI accession no.
UniProt accession no.
shipped in
wet ice
target post-translational modification
unmodified
Gene Information
human ... MAGI2(9863)
General description
The spatial coordination of neurotransmitter receptors with other postsynaptic signaling and structural molecules is regulated by a diverse array of cell-specific scaffolding proteins. The synaptic trafficking of AMPA receptors by the stargazin protein in some neurons, for example, depends on specific interactions between the C terminus of stargazin and the PDZ [postsynaptic density-95 (PSD-95)/Discs large/zona occludens-1] domains of membrane-associated guanylate kinase scaffolding proteins PSD-93 or PSD-95. Stargazin [Cacng2 (Ca2+ channel gamma2 subunit)] is one of four closely related proteins recently categorized as transmembrane AMPA receptor regulating proteins (TARPs) that appear to share similar functions but exhibit distinct expression patterns in the CNS. MAGI-2 (membrane associated guanylate kinase, WW and PDZ domain containing 2) has been identified as a novel candidate interactor with the cytoplasmic C termini of the TARPs. MAGI-2 [also known as S-SCAM (synaptic scaffolding molecule)] is a multi-PDZ domain scaffolding protein that interacts with several different ligands in brain, including PTEN (phosphatase and tensin homolog), dasm1 (dendrite arborization and synapse maturation 1), dendrin, axin, beta- and delta-catenin, neuroligin, hyperpolarization-activated cation channels, beta1-adrenergic receptors, and NMDA receptors. MAGI-2 coimmunoprecipitates with stargazin in vivo. Expression of stargazin recruites MAGI-2 to cell membranes and cell-cell contact sites in transfected HEK-293T cells dependent on the presence of the stargazin -TTPV motif. MAGI-2 represents a strong candidate for linking TARP/AMPA receptor complexes to a wide range of other postsynaptic molecules and pathways and advance our knowledge of protein interactions at mammalian CNS synapses.
Specificity
Cat. # AB9878 recognizes MAGI-2
Reactivity with other species has not been determined.
Immunogen
GST-tagged MAGI-2
Application
This Anti-MAGI-2 Antibody is validated for use in IC, IH, IP, WB for the detection of MAGI-2.
Quality
Routinely evaluated by Western Blotting on mouse brain tissue lysate.
Western Blotting Analysis: 1:500 dilution of this lot was used to detect MAGI-2 on mouse brain lysate.
Western Blotting Analysis: 1:500 dilution of this lot was used to detect MAGI-2 on mouse brain lysate.
Target description
~140 kDa
Physical form
Format: Purified
Purified antibody in 0.1M Tris-Glycine (pH7.4) 15mM NaCl with 0.05% NaN3.
Storage and Stability
Stable for 1 year at 2-8°C from date of receipt.
Handling Recommendations: Prior to removing the cap, gently centrifuge the vial and gently mix the solution.
Handling Recommendations: Prior to removing the cap, gently centrifuge the vial and gently mix the solution.
Analysis Note
Control
Mouse brain
Mouse brain
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Legal Information
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
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Storage Class Code
12 - Non Combustible Liquids
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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Naomi Tanga et al.
Glia, 67(5), 967-984 (2019-01-23)
Protein tyrosine phosphatase receptor type Z (PTPRZ) maintains oligodendrocyte precursor cells (OPCs) in an undifferentiated state. The inhibition of PTPase by its ligand pleiotrophin (PTN) promotes OPC differentiation; however, the substrate molecules of PTPRZ involved in the differentiation have not
Naomi Tanga et al.
Glia, 67(5), 967-984 (2019-01-23)
Protein tyrosine phosphatase receptor type Z (PTPRZ) maintains oligodendrocyte precursor cells (OPCs) in an undifferentiated state. The inhibition of PTPase by its ligand pleiotrophin (PTN) promotes OPC differentiation; however, the substrate molecules of PTPRZ involved in the differentiation have not
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