AB951-I
Anti-Neuron Specific Enolase Antibody
from rabbit
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Gamma-enolase, 2-phospho-D-glycerate hydro-lyase, Enolase 2, Neural enolase, Neuron-specific enolase, NSE
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biological source
rabbit
Quality Level
antibody form
purified antibody
antibody product type
primary antibodies
clone
polyclonal
species reactivity
human, mouse, rat
species reactivity (predicted by homology)
bovine (based on 100% sequence homology)
technique(s)
immunohistochemistry: suitable (paraffin)
western blot: suitable
NCBI accession no.
UniProt accession no.
shipped in
wet ice
target post-translational modification
unmodified
Gene Information
human ... ENO2(2026)
General description
The glycolytic enzyme enolase (2-phospho-D-glycerate hydrolase, E.C. 4.2.1.11) is composed of two subunits and exists in three molecular forms in human brain: non-neuronal enolase (NNE; alpha, alpha′-enolase), neuron-specific enolase (NSE; gamma,gamma′-enolase, also known as 14-3-2 protein and NSP) and the short-lived heterodimer alpha, gamma′-enolase. The neuron specific form is a highly acidic homodimeric protin of 78kDa, which has been found in central and peripheral nervous elements and also in many neuroendocrine cells and derivative tumors. NSE is regarded as the best described serum marker for small cell lung cancer. In addition, the measurement of NSE levels in both serum and cerebrospinal fluid following cerebral ischaemia and traumatic head injury provides a reliable laboratory indicator of the degree of brain cell damage and may allow early prediction of prognostic outcome.
Specificity
This antibody recognizes a region near the N-terminus of Neuron Specific Enolase.
Immunogen
Epitope: Near N-terminus
KLH-conjugated linear peptide corresponding to the near N-terminus of human Neuron Specific Enolase.
Application
Anti-Neuron Specific Enolase Antibody is a highly specific rabbit polyclonal antibody, that targets Neuron-Specific Enolase & has been tested in western blotting & IHC (Paraffin).
Immunohistochemistry Analysis: A 1:100 dilution from a representative lot detected in Neuron Specific Enolase in human thalamus tissue and glioma sections.
Research Category
Neuroscience
Neuroscience
Research Sub Category
Developmental Neuroscience
Developmental Neuroscience
Quality
Evaluated by Western Blotting in rat brain tissue lysate.
Western Blotting Analysis: 1 µg/mL of this antibody detected Neuron Specific Enolase in 10 µg of rat brain tissue lysate.
Western Blotting Analysis: 1 µg/mL of this antibody detected Neuron Specific Enolase in 10 µg of rat brain tissue lysate.
Target description
~47 kDa observed. Cat. No. AB951, Anti-Neuron Specific Enolase detects Alpha Enolase (ENO1) at ~39kDa However, Neuron Specific Enolase has been observed at ~48 kDa (Braus, B., K., et al (2008). Vet Immunol Immunopathol 124(1-2):177-183.; Feo, S., et al. (2000). FEBS Lett. 473(1):47-52.).
Linkage
Replaces: AB951
Physical form
Format: Purified
Protein A purified
Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Storage and Stability
Stable for 1 year at 2-8°C from date of receipt.
Analysis Note
Control
Rat brain tissue lysate
Rat brain tissue lysate
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Regulatory Information
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Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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