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CD138 antigen, heparan sulfate proteoglycan fibroblast growth factor
receptor, syndecan 1, syndecan proteoglycan 1
receptor, syndecan 1, syndecan proteoglycan 1
Recommended Products
biological source
rabbit
Quality Level
antibody form
serum
antibody product type
primary antibodies
clone
polyclonal
species reactivity
mouse, human
technique(s)
western blot: suitable
NCBI accession no.
UniProt accession no.
shipped in
wet ice
target post-translational modification
unmodified
Gene Information
human ... SDC1(6382)
General description
Syndecan-1, also known as CD138, is a transmembrane (Type I) heparan sulfate proteoglycan and is a member of the syndecan proteoglycan family. The syndecans mediate cell binding, cell signaling, and cytoskeletal organization. Bearing properties of both heparan sulfate and chondroitin sulfate, Syndecan-1 functions as a cell surface proteoglycan membrane protein and participates in cell proliferation, cell migration and cell-matrix interactions via its receptor for extracellular matrix proteins. Altered syndecan-1 expression has been detected in several different tumor types.
Specificity
Cat. No. AB8230 recognizes mouse Syndecan-1.
Predicted to cross react with human, based on 100% sequence homology.
Reactivity with other species has not been tested.
Immunogen
His-tagged, full-length human recombinant Syndecan-1 protein.
Application
Anti-Syndecan-1 Antibody is an antibody against Syndecan-1 for use in WB.
Research Category
Cell Structure
Cell Structure
Research Sub Category
ECM Proteins
ECM Proteins
Quality
Evaluated on a representative lot by Western blot on mouse liver lysate using 1:1000 dilution of Anti-Syndecan-1.
Target description
The expected molecular weight of monomeric Syndecan-1 is ~32 kDa; however, the core protein often migrates as an SDS-stable dimer with an approximate M.W. of 77 kDa Additionally, a high M.W. form of the protein may often be seen between 500-1000 kDa, likely the mature form of Syndecan-1, with all of its heparan and chondroitin sulfate additions. In whole tissues, this mature form often binds tightly to ECM molecules and may require solublization by urea extraction for additional SDS-PAGE analysis (Wilsie, 2006). Triton X-100 or similar mild detergents are sufficient to release the immature precursor forms of Syndecan-1.
Physical form
Rabbit serum containing 0.05% NaN3.
Storage and Stability
Maintain at -20°C for up to 1 year from date of receipt.
Analysis Note
Control
Mouse liver lysate
Mouse liver lysate
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
WGK
WGK 1
Certificates of Analysis (COA)
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