AB769
Anti-Collagen Type IV Antibody
Chemicon®, from goat
Synonym(s):
Anti-Anti-BSVD, Anti-Anti-BSVD1, Anti-Anti-COL4A1s, Anti-Anti-PADMAL, Anti-Anti-RATOR
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About This Item
UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
biological source
goat
Quality Level
antibody form
affinity purified immunoglobulin
antibody product type
primary antibodies
clone
polyclonal
purified by
affinity chromatography
species reactivity
human, bovine, mouse
manufacturer/tradename
Chemicon®
technique(s)
ELISA: suitable
dot blot: suitable
immunocytochemistry: suitable
immunohistochemistry: suitable
suitability
not suitable for Western blot
not suitable for immunohistochemistry (Paraffin)
NCBI accession no.
UniProt accession no.
shipped in
wet ice
target post-translational modification
unmodified
Gene Information
human ... COL4A1(1282)
Specificity
Recognizes Human and bovine type IV collagen as demonstrated by ELISA. Less than 10% cross reactivity with collagen types I, II, III, V, and VI. May show reactivity to type IV collagen from other species. AB769 has not been tested with other extracellular matrix proteins (e.g., laminin, fibronectin).
Immunogen
Human and bovine placental collagen type IV
Application
Detect Collagen Type IV using this Anti-Collagen Type IV Antibody validated for use in DB, ELISA, IC, IH.
Dot and slot blotting: 1:100-1:500
ELISA: 1:1000-1:4000
Indirect immunohistochemistry (frozen sections only): 1:10-1:40
Immunocytochemistry 1:10-1:20
Optimal working dilutions must be determined by the end user.
ELISA: 1:1000-1:4000
Indirect immunohistochemistry (frozen sections only): 1:10-1:40
Immunocytochemistry 1:10-1:20
Optimal working dilutions must be determined by the end user.
Research Category
Cell Structure
Cell Structure
Research Sub Category
ECM Proteins
ECM Proteins
Physical form
Affinity Purified immunoglobulin. Prior to purification the antisera was adsorbed against collagen type I, II, III, V and VI immobilized on Sepharose™ 4B. Liquid in borate buffered saline, pH 8.2, no sodium azide.
ImmunoAffinity Purified
Storage and Stability
Maintain for 1 year at 2–8°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Analysis Note
Control
Positive Control: Kidney, muscle, tendon spleen tissue
Negative Control: Neurons/glia
Positive Control: Kidney, muscle, tendon spleen tissue
Negative Control: Neurons/glia
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Legal Information
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Sepharose is a trademark of Cytiva
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Signal Word
Danger
Hazard Statements
Precautionary Statements
Hazard Classifications
Repr. 1B
WGK
nwg
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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