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Merck
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AB758B

Anti-Collagen Type I Antibody, biotin conjugated

Chemicon®, from goat

Synonym(s):

Anti-CAFYD, Anti-EDSARTH1, Anti-EDSC, Anti-OI1, Anti-OI2, Anti-OI3, Anti-OI4

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About This Item

UNSPSC Code:
12352203
NACRES:
NA.41
eCl@ss:
32160702

Key Documents

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Product Name

Anti-Collagen Type I Antibody, biotin conjugated, Chemicon®, from goat

biological source

goat

conjugate

biotin conjugate

antibody form

affinity purified immunoglobulin

antibody product type

primary antibodies

clone

polyclonal

species reactivity

bovine, human

manufacturer/tradename

Chemicon®

technique(s)

ELISA: suitable
dot blot: suitable
immunohistochemistry: suitable
western blot: suitable

suitability

not suitable for immunohistochemistry (Paraffin)

NCBI accession no.

NM_000088.3

UniProt accession no.

P02452

shipped in

wet ice

target post-translational modification

unmodified

Quality Level

100

Gene Information

human ... COL1A1(1277)

Application

Anti-Collagen Type I Antibody, biotin conjugated is an antibody against Collagen Type Id for use in DB, ELISA, WB, IH.
Dot and slot blotting: 1:500-1:1,000

ELISA: 1:1,000-4,000

Indirect immunohistochemistry (frozen sections only): 1:20-1:40

The non-biotinylated version (Cat. # AB758) reacts with human collagen I in reduced westerns with supernatant samples from serum-free cultures of human fibroblasts , and has been cited for use in WB applications (PMID: 20089870)

Not recommended for paraffin tissue sections.

Optimal working dilutions must be determined by the end user.
Research Category
Cell Structure
Research Sub Category
ECM Proteins

Biochem/physiol Actions

Human type I collagen as demonstrated by ELISA. Less than 10% cross reactivity with collagen types II, III, IV, V, and VI. May show reactivity to type I collagen from other species. AB758B has not been tested with other extracellular matrix proteins (e.g., laminin, fibronectin).

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Immunogen

Human placental collagen type I

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Physical form

Affinity purified immunoglobulin, biotin labeled. Prior to purification the antisera was adsorbed against collagen type II, III, IV, V and VI immobilized on Sepharose™ 4B.. Liquid in phosphate buffered saline, pH 7.4, containing 0.1% sodium azide.

Preparation Note

Maintain at 2-8°C in undiluted aliquots for up to 12 months.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Sepharose is a trademark of Cytiva

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Storage Class

12 - Non Combustible Liquids

wgk

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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