All Photos(2)
Synonym(s):
microtubule-associated protein, RP/EB family, member 1, adenomatous polyposis coli-binding protein EB1, microtubule-associated protein RP/EB family member 1, End-binding protein 1, APC-binding protein EB1
UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Recommended Products
biological source
rabbit
antibody form
purified antibody
antibody product type
primary antibodies
clone
polyclonal
species reactivity
human
species reactivity (predicted by homology)
rat (based on 100% sequence homology), mouse (based on 100% sequence homology)
technique(s)
immunocytochemistry: suitable
western blot: suitable
NCBI accession no.
UniProt accession no.
shipped in
wet ice
target post-translational modification
unmodified
Gene Information
human ... MAPRE1(22919)
General description
End binding protein 1 (EB1) is thought to be associated with adenomatous polyposis coli (APC) and may play a part in regulation of microtubule dynamics in the mammalian cells. EB1 could also play a role in the regulation of microtubule assembly and stability. EB1 is essential for proper cilia assembly in fibroblasts. EB1 has been shown to excite spontaneous nucleation and microtubule growth. APC gene mutation and the loss of negatively regulating WNT signaling is thought to play a role in the proliferation changes of cancer. The loss of association with EB1 could contribute to the development of certain cancers.
Specificity
This antibody recognizes EB1 at the C-terminus.
EB1 is 67% homologous to EB3.
EB1 is 67% homologous to EB3.
Immunogen
Epitope: C-terminus
KLH-conjugated linear peptide corresponding to the C-terminus of human EB1.
Application
Anti-EB1 Antibody is an antibody against EB1 for use in WB & IC.
Immunocytochemistry Analysis: A 1:500 dilution from a previous lot detected EB1 in NIH/3T3, A431, and HeLa cells.
Research Category
Cell Structure
Cell Structure
Research Sub Category
Cytoskeletal Signaling
Cytoskeletal Signaling
Quality
Evaluated by Western Blot in HeLa cell lysate.
Western Blot Analysis: 0.5 µg/mL of this antibody detected EB1 in 10 µg of HeLa cell lysate.
Western Blot Analysis: 0.5 µg/mL of this antibody detected EB1 in 10 µg of HeLa cell lysate.
Target description
~32 kDa observed.
An uncharacterized band appears at ~52 kDa
An uncharacterized band appears at ~52 kDa
Physical form
Format: Purified
Protein A purified
Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Storage and Stability
Stable for 1 year at 2-8°C from date of receipt.
Analysis Note
Control
HeLa cell lysate
HeLa cell lysate
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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Inyoung Lee et al.
The Journal of biological chemistry, 292(39), 16267-16276 (2017-08-23)
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