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AB6057

Sigma-Aldrich

Anti-EB1 Antibody

from rabbit

Synonym(s):

microtubule-associated protein, RP/EB family, member 1, adenomatous polyposis coli-binding protein EB1, microtubule-associated protein RP/EB family member 1, End-binding protein 1, APC-binding protein EB1

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

biological source

rabbit

antibody form

purified antibody

antibody product type

primary antibodies

clone

polyclonal

species reactivity

human

species reactivity (predicted by homology)

rat (based on 100% sequence homology), mouse (based on 100% sequence homology)

technique(s)

immunocytochemistry: suitable
western blot: suitable

NCBI accession no.

NP_036457

UniProt accession no.

Q15691

shipped in

wet ice

target post-translational modification

unmodified

Gene Information

human ... MAPRE1(22919)

General description

End binding protein 1 (EB1) is thought to be associated with adenomatous polyposis coli (APC) and may play a part in regulation of microtubule dynamics in the mammalian cells. EB1 could also play a role in the regulation of microtubule assembly and stability. EB1 is essential for proper cilia assembly in fibroblasts. EB1 has been shown to excite spontaneous nucleation and microtubule growth. APC gene mutation and the loss of negatively regulating WNT signaling is thought to play a role in the proliferation changes of cancer. The loss of association with EB1 could contribute to the development of certain cancers.

Specificity

This antibody recognizes EB1 at the C-terminus.
EB1 is 67% homologous to EB3.

Immunogen

Epitope: C-terminus
KLH-conjugated linear peptide corresponding to the C-terminus of human EB1.

Application

Anti-EB1 Antibody is an antibody against EB1 for use in WB & IC.
Immunocytochemistry Analysis: A 1:500 dilution from a previous lot detected EB1 in NIH/3T3, A431, and HeLa cells.
Research Category
Cell Structure
Research Sub Category
Cytoskeletal Signaling

Quality

Evaluated by Western Blot in HeLa cell lysate.

Western Blot Analysis: 0.5 µg/mL of this antibody detected EB1 in 10 µg of HeLa cell lysate.

Target description

~32 kDa observed.
An uncharacterized band appears at ~52 kDa

Physical form

Format: Purified
Protein A purified
Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Analysis Note

Control
HeLa cell lysate

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Sarah J Benbow et al.
Neurotoxicity research, 32(1), 151-162 (2017-04-10)
Chemotherapy-induced peripheral neuropathy (CIPN) is a common side effect of anticancer treatment with microtubule-targeted agents (MTAs). The frequency of severe CIPN, which can be dose limiting and even life threatening, varies widely among different MTAs. For example, paclitaxel induces a
Inyoung Lee et al.
The Journal of biological chemistry, 292(39), 16267-16276 (2017-08-23)
The centrosome serves as a major microtubule-organizing center (MTOC). The Cdc6 protein is a component of the pre-replicative complex and a licensing factor for the initiation of chromosome replication and localizes to centrosomes during the S and G2 phases of

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