AB6039
Anti-Vinculin Antibody
from rabbit, purified by affinity chromatography
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Synonym(s):
Metavinculin
UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Recommended Products
biological source
rabbit
antibody form
affinity isolated antibody
antibody product type
primary antibodies
clone
polyclonal
purified by
affinity chromatography
species reactivity
rat, human, mouse
technique(s)
western blot: suitable
NCBI accession no.
UniProt accession no.
shipped in
wet ice
target post-translational modification
unmodified
Gene Information
human ... VCL(7414)
General description
Cell spreading and movement occur though the process of binding of cell surface integrin receptors to extracellular matrix adhesion molecules. In mammalian cells, vinculin is a membrane-cytoskeletal protein in focal adhesion plaques that is involved in linkage of integrin adhesion molecules to the actin cytoskeleton. More specifically, vinculin binds to talin at its N-terminus, which, in turn, binds to beta integrins. At its C-terminus, vinculin binds to actin, phospholipids, and paxillin-forming homodimers. The binding complex of vinculin to talin and actin serves to anchor actin filaments to the membrane, (Ezzell, 1997).
Vinculin′s ability to interact with integrins at the focal adhesion appears to be critical for control of cytoskeletal mechanics, cell spreading, and lamellipodia formation. Thus, vinculin appears to play a key role in shape control based on its ability to modulate focal adhesion structure and function.
Vinculin′s ability to interact with integrins at the focal adhesion appears to be critical for control of cytoskeletal mechanics, cell spreading, and lamellipodia formation. Thus, vinculin appears to play a key role in shape control based on its ability to modulate focal adhesion structure and function.
Specificity
Predicted to cross react with monkey, pig, cow, and horse based on sequence homology.
The antibody recognizes an internal domain of human vinculin.
Immunogen
Epitope: Internal Domain
KLH-conjugated synthetic peptide corresponding to an internal domain of human Vinculin.
Application
Detect Vinculin using this Anti-Vinculin Antibody validated for use in WB.
Research Category
Cell Structure
Cell Structure
Research Sub Category
Cytoskeletal Signaling
Cytoskeleton
Cytoskeletal Signaling
Cytoskeleton
Quality
Evaluated on a representative lot by Western blot on HepG2 cell lysate using Anti-Vinculin.
Target description
~ 124 kDa
Physical form
Antigen Affinity Purified
Purified in buffer containing 0.1 M Tris-Glycine, pH 7.4, 150 mM NaCl with 0.05% sodium azide.
Storage and Stability
Stable for 1 year at 2-8°C from date of receipt.
Analysis Note
Control
HepG2 cell lysate.
HepG2 cell lysate.
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Product No.
Description
Pricing
WGK
WGK 1
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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