AB5818
Anti-Potassium Channel Kir4.1 Antibody
Chemicon®, from rabbit
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About This Item
UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
biological source
rabbit
antibody form
affinity purified immunoglobulin
antibody product type
primary antibodies
clone
polyclonal
purified by
affinity chromatography
species reactivity
rat, mouse, human
manufacturer/tradename
Chemicon®
technique(s)
western blot: suitable
NCBI accession no.
UniProt accession no.
shipped in
wet ice
target post-translational modification
unmodified
Gene Information
human ... KCNJ10(3766)
Specificity
Recognizes Kir4.1. The epitope does not share homology with any other known proteins.
SPECIES REACTIVITIES: The antibody is also expected to work with mouse and human due to sequence homology. Other species have not been tested.
SPECIES REACTIVITIES: The antibody is also expected to work with mouse and human due to sequence homology. Other species have not been tested.
Immunogen
CONTROL ANTIGEN: Included free of charge with the antibody is 20 μg of control antigen (lyophilized powder). The stock solution of the antigen can be made up using 100 μL of sterile distilled water. For negative control, preincubate 1 μg of protein with 1 μg of antibody for one hour at room temperature. Optimal concentrations must be determined by the end user. Synthetic peptide corresponding to amino acids 356-375 of rat, mouse or human Kir4.1 (Accession P49655).
Application
This Anti-Potassium Channel Kir4.1 Antibody is validated for use in WB for the detection of Potassium Channel Kir4.1.
Western blot: 1:200-1:600 using ECL on rat brain membranes or human platelets.
Dilutions should be made using a carrier protein such as BSA (1-3%)
Optimal working dilutions must be determined by the end user.
Dilutions should be made using a carrier protein such as BSA (1-3%)
Optimal working dilutions must be determined by the end user.
Physical form
Affinity purified immunoglobulin. Lyophilized from phosphate buffered saline, pH 7.4, containing 1% BSA, 5% sucrose as a stabilizer and 0.025% sodium azide as a preservative. Reconstitute with 50 μL of sterile deionized water. Centrifuge antibody preparation before use (10,000 xg for
Analysis Note
Control
Included free of charge with the antibody is 40 μg of control antigen (lyophilized powder). The stock solution of the antigen can be made up using 100 μL of sterile distilled water. For negative control, preincubate 1 μg of protein with 1 μg of antibody for one hour at room temperature. Optimal concentrations must be determined by the end user.
Included free of charge with the antibody is 40 μg of control antigen (lyophilized powder). The stock solution of the antigen can be made up using 100 μL of sterile distilled water. For negative control, preincubate 1 μg of protein with 1 μg of antibody for one hour at room temperature. Optimal concentrations must be determined by the end user.
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Legal Information
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
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Hazard Statements
Precautionary Statements
Hazard Classifications
Aquatic Chronic 3
Storage Class Code
11 - Combustible Solids
WGK
WGK 3
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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