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Merck
CN

AB5674P

Anti-Acid Sensitive Ion Channel 1 Antibody

Chemicon®, from rabbit

Synonym(s):

ASIC1, BNC1, MDEG, BNaC1a, Amiloride-sensitive Cation Channel 2

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About This Item

UNSPSC Code:
12352203
NACRES:
NA.41
eCl@ss:
32160702

Key Documents

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Product Name

Anti-Acid Sensitive Ion Channel 1 Antibody, Chemicon®, from rabbit

biological source

rabbit

conjugate

unconjugated

antibody form

affinity purified immunoglobulin

antibody product type

primary antibodies

clone

polyclonal

purified by

affinity chromatography

species reactivity

rat, human

manufacturer/tradename

Chemicon®

technique(s)

ELISA: suitable
western blot: suitable

NCBI accession no.

NM_001095.2
NM_020039.2

UniProt accession no.

P78348

shipped in

dry ice

target post-translational modification

unmodified

Quality Level

100

Physical form

Affinity purified immunoglobulin. Liquid in phosphate buffered saline containing 0.1% BSA.

Preparation Note

Maintain at -20°C in undiluted aliquots for up to 6 months. Avoid repeated freeze/thaw cycles.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Application

Detect Acid Sensitive Ion Channel 1 using this Anti-Acid Sensitive Ion Channel 1 Antibody validated for use in ELISA & WB.
Research Category
Neuroscience
Research Sub Category
Ion Channels & Transporters
Western blot: 1-10 μg/mL using ECL.

Immunohistochemistry: not tested. It is recommended that the antibody be tried at 1-20 μg/mL on paraformaldehyde fixed tissue.

ELISA: 0.5-1 μg/mL using 1 μg/mL control peptide (Catalog number AG394) per well.

Optimal working dilutions must be determined by the end user.

Biochem/physiol Actions

Recognizes rat Acid Sensitive Ion Channel 1 (ASIC1). The immunogen shows no significant sequence homology with ASIC-beta or other ASICs.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Immunogen

A 20 amino acid peptide sequence near the extracellular N-terminus of rat ASIC1 (Chen et al. 1998; Waldmann et al. 1997; Garcia-Anoveros et al. 1997; Waldmann et al. 1996).

The immunogen sequence has 100% homology with human sodium channel 2 or BNaC2.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

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Storage Class

12 - Non Combustible Liquids

wgk

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Selina M Garcia et al.
The Journal of general physiology, 155(2) (2022-12-10)
Acid-sensing ion channel 1a (ASIC1a) belongs to a novel family of proton-gated cation channels that are permeable to both Na+ and Ca2+. ASIC1a is expressed in vascular smooth muscle and endothelial cells in a variety of vascular beds, yet little
Neil D Detweiler et al.
Journal of applied physiology (Bethesda, Md. : 1985), 127(2), 393-407 (2019-06-07)
Acid-sensing ion channels (ASICs) are voltage-insensitive cation channels that contribute to cellular excitability. We previously reported that ASIC1 in pulmonary artery smooth muscle cells (PASMC) contribute to pulmonary vasoreactivity and vascular remodeling during the development of chronic hypoxia (CH)-induced pulmonary
Selina M Garcia et al.
Frontiers in molecular biosciences, 9, 989809-989809 (2022-10-25)
Acid-sensing ion channel 1a (ASIC1a) is a voltage-independent, non-selective cation channel that conducts both Na+ and Ca2+. Activation of ASIC1a elicits plasma membrane depolarization and stimulates intracellular Ca2+-dependent signaling pathways in multiple cell types, including vascular smooth muscle (SM) and
Selina M Garcia et al.
PloS one, 15(7), e0236288-e0236288 (2020-07-24)
Although voltage-gated Ca2+ channels (VGCC) are a major Ca2+ entry pathway in vascular smooth muscle cells (VSMCs), several other Ca2+-influx mechanisms exist and play important roles in vasoreactivity. One of these is store-operated Ca2+ entry (SOCE), mediated by an interaction

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