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AB5535-AF555

Sigma-Aldrich

Anti-Sox9 Antibody, Alexa Fluor™ 555 conjugate

from rabbit, ALEXA FLUOR™ 555

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Synonym(s):
Transcription factor SOX-9, Sox9
UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

biological source

rabbit

Quality Level

100

conjugate

ALEXA FLUOR™ 555

antibody form

affinity purified immunoglobulin

antibody product type

primary antibodies

clone

polyclonal

species reactivity

human, mouse, rat

species reactivity (predicted by homology)

horse (based on 100% sequence homology), sheep (based on 100% sequence homology), feline (based on 100% sequence homology), bovine (based on 100% sequence homology)

technique(s)

immunocytochemistry: suitable

NCBI accession no.

NP_000337

UniProt accession no.

P48436

shipped in

wet ice

target post-translational modification

unmodified

Gene Information

human ... SOX9(6662)

General description

Transcription factor SOX-9 (UniProt P48436; also known as Sex-determining region Y-box 9 protein, SRY-related HMG-box gene 9) is encoded by the SOX9 (also known as CMD1, CMPD1, SRA1) gene (Gene ID 6662) in human. Sox family of genes are related to the mammalian sex determining gene SRY. These genes contain sequences that encode for the HMG-box domains that mediate sequence-specific DNA binding. Sox genes encode transcriptional regulators involved in cell fate decision during development and the control of diverse developmental processes. The highly complex group of Sox genes cluster at at least 40 genomic loci and at least 30 Sox genes have been identified.

Immunogen

Epitope: C-terminus
KLH-conjugated linear peptide corresponding to the C-terminus of human Sox9.

Application

Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected Sox9 in HepG2 cells.
The unconjugated version (Cat. No. AB5535) is also shown to be suitable for Western blotting and immunohistochemistry applications.
Research Category
Epigenetics & Nuclear Function
Research Sub Category
Nuclear Receptors
This Anti-Sox9 Antibody, Alexa Fluor™ 555 conjugate is validated for use in Immunocytochemistry for the detection of Sox9.

Quality

Evaluated by Immunocytochemistry in HeLa cells.

Immunocytochemistry Analysis: A 1:100 dilution of this antibody detected Sox9 in HeLa cells.

Target description

55-65 kDa observed

Physical form

Affinity purified
Purified rabbit conjugate antibody in PBS with 15 mg/ml BSA and 0.1% sodium azide.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Other Notes

Concentration: Please refer to lot specific datasheet.

Legal Information

ALEXA FLUOR is a trademark of Life Technologies

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Xiaomei Xie et al.
FASEB journal : official publication of the Federation of American Societies for Experimental Biology, 37(5), e22918-e22918 (2023-04-12)
Sertoli cells are essential for testis development and normal spermatogenesis by providing support and nutrients. Pre-messenger RNA (pre-mRNA) processing is the basic mechanism required for gene expression, and members of the serine/arginine-rich protein (SR) family are key components of the

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