Anti-Vesicular Glutamate Transporter 3 (VGLUT3) Antibody

Vesicular glutamate transporter 3 (UniProt Q7TSF2; also known as Solute carrier family 17 member 8, VGluT3) is encoded by the Slc17a8 (also known as Vglut3) gene (Gene ID 266767) in rat species. Vesicular glutamate transporters (VGluTs) are proton-dependent carriers responsible for the upload of the major excitatory neurotransmitter glutamate into synaptic vesicles within presynaptic terminals to allow later regulated release at the synaptic cleft. Studies conducted in mice show that VGluT1 and VGluT2 are essential for vital functions, while Vglut3-knockout mice have a normal life expectancy despite being deaf and developing anxiety and discrete basal locomotor phenotypes. VGluT1- and VGluT2-expressing neurons represent the bulk of canonical glutamatergic neurons in the brain, with VgluT1 representing the major subtype and accounting for approximately 80% of total vesicular transport of glutamate in the brain. In contrast, VgluT3 is found in discrete population of non-glutamatergic neurons using other transmitters than glutamate, such as cholinergic interneurons in the dorsal and ventral striatum, subpopulations of GABAergic basket cells in the cortex and hippocampus, and serotoninergic neurons, where it is involved in more subtle modulation of local transmission. Rat VGluT3 is a 12-transmembrane (a.a. 77-97, 131-151, 154-174, 183-203, 222-242, 250-270, 315-335, 354-374, 391-411, 414-434, 448-468, 482-502) protein with 7 cytoplasmic and 6 vacuolar domains, having both its N- and C-terminal tails (a.a. 1-76 and 503-585) exposed at the cytoplasmic side.

This polyclonal guinea pig antiserum was raised against a VGluT3 C-terminal cytoplasmic domain sequence conserved between rat and murine species and shares 96% homology with the corresponding human sequence.

Epitope: C-terminal cytoplasmic domain.

KLH-conjugated linear peptide corresponding to a C-terminal cytoplasmic domain sequence of rat vesicular glutamate transporter 3 (VGluT3).

Anti-Vesicular Glutamate Transporter 3 (VGLUT3) is an antibody against Vesicular Glutamate Transporter 3 (VGLUT3) for use in Immunocytochemistry and Western Blotting.

Immunocytochemistry Analysis: A 1:250 dilution from a representative lot detected vesicular glutamate transporter 3 (VGluT3) in PC12 rat pheochromocytoma cells.

Research Category
Neuroscience

Research Sub Category
Neurotransmitters & Receptors

Evaluated by Western Blotting in PC12 cell lysate.

Western Blotting Analysis: A 1:2,000 dilution of this antibody detected vesicular glutamate transporter 3 (VGluT3) in 10 µg of PC12 rat pheochromocytoma cell lysate.

~61 kDa observed. 64.76 kDa (rat), 66.15 kDa (mouse), and 64.99/59.08 kDa (human isoform 1/2) calculated. A ~130 kDa central nervous system VGluT3 isoform has also been described (Munguba, G.C., et al. (2011). Mol. Vis. 17:413-419). Uncharacterized band(s) may appear in some lysates.

Replaces: AB5421

Guinea pig polyclonal antibody serum with 0.05% sodium azide.

Unpurified

Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Concentration: Please refer to lot specific datasheet.

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