AB5208
Anti-Sodium Channel Antibody, Voltage Gated, Brain Type III, pain
Chemicon®, from rabbit
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Synonym(s):
Nav1.3, SCN3A
UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Recommended Products
biological source
rabbit
Quality Level
antibody form
affinity purified immunoglobulin
antibody product type
primary antibodies
clone
polyclonal
purified by
affinity chromatography
species reactivity
rat, mouse
manufacturer/tradename
Chemicon®
technique(s)
immunohistochemistry: suitable
western blot: suitable
NCBI accession no.
UniProt accession no.
shipped in
wet ice
target post-translational modification
unmodified
Gene Information
human ... SCN3A(6328)
Specificity
Recognizes Nav1.3 (type III a subunit of VGSC, Scn3a). Does not cross react with any other sodium channel antigens tested so far.
Immunogen
Purified peptide of alpha subunit (amino acids 511-524) of rat Nav1.3
( type III voltage-gated sodium channel VGSC)
( type III voltage-gated sodium channel VGSC)
Application
All procedures that are going to receive a full-length protein should be performed at 4°C, and the following protease inhibitor mixture should be used: pepstatin A (1 μg/mL), leupeptin (1 μg/mL), aprotinin (1 μg/mL), Pefabloc SC (0.2 mM), benzamidine (0.1 mg/mL), and calpain inhibitors I and II (8 μg/mL each).
Western blot: 1:200 using ECL on newborn rat brain membranes.
Dilutions should be made using a carrier protein such as BSA (1-3%).
Immunohistochemistry: on fixed frozen sections.
Optimal working dilutions must be determined by the end user.
Western blot: 1:200 using ECL on newborn rat brain membranes.
Dilutions should be made using a carrier protein such as BSA (1-3%).
Immunohistochemistry: on fixed frozen sections.
Optimal working dilutions must be determined by the end user.
Detect Sodium Channel using this Anti-Sodium Channel Antibody, Voltage Gated, Brain Type III, pain validated for use in IH & WB.
Research Category
Neuroscience
Neuroscience
Research Sub Category
Ion Channels & Transporters
Ion Channels & Transporters
Physical form
Affinity purified immunoglobulin. Lyophilized from phosphate buffered saline, pH 7.4, containing 1% BSA as a stabilizer and 0.05% sodium azide as a preservative. Reconstitute with 50 mL of sterile deionized water. Centrifuge antibody preparation before use (10,000 xg for 5 min).
Storage and Stability
Maintain lyophilized material at -20°C for up to 12 months after date of receipt. After reconstitution maintain at -20°C in undiluted aliquots for up to 6 months. Avoid repeated freeze/thaw cycles.
Analysis Note
Control
CONTROL ANTIGEN: Included free of charge with the antibody is 40 μg of control antigen (lyophilized powder). The stock solution of the antigen can be made up using 100 μL of sterile deionized water. For negative control, preincubate 0.75 μg of peptide with 1 μg of antibody for one hour at room temperature. Optimal concentrations must be determined by the end user.
CONTROL ANTIGEN: Included free of charge with the antibody is 40 μg of control antigen (lyophilized powder). The stock solution of the antigen can be made up using 100 μL of sterile deionized water. For negative control, preincubate 0.75 μg of peptide with 1 μg of antibody for one hour at room temperature. Optimal concentrations must be determined by the end user.
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Legal Information
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Hazard Statements
Precautionary Statements
Hazard Classifications
Aquatic Chronic 3
WGK
WGK 3
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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Na Gao et al.
BMC neuroscience, 10, 20-20 (2009-03-17)
Taste bud cells transmit information regarding the contents of food from taste receptors embedded in apical microvilli to gustatory nerve fibers innervating basolateral membranes. In particular, taste cells depolarize, activate voltage-gated sodium channels, and fire action potentials in response to
Florian Bolz et al.
Frontiers in neuroanatomy, 11, 28-28 (2017-04-20)
To understand the molecular basis of neuronal excitation in the mammalian olfactory system, we conducted a systematic analysis of the organization of voltage-gated sodium (Nav) channel subunits in the main olfactory epithelium (MOE) and vomeronasal organ (VNO) of adult mice.
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