AB5062P
Anti-Vesicular GABA Transporter (VGAT) Antibody
Chemicon®, from rabbit
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Synonym(s):
Vesicular Inhibitory Amino Acid Transporter
UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
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biological source
rabbit
Quality Level
antibody form
affinity isolated antibody
antibody product type
primary antibodies
clone
polyclonal
purified by
affinity chromatography
species reactivity
mouse, monkey, human, rat
manufacturer/tradename
Chemicon®
technique(s)
ELISA: suitable
immunofluorescence: suitable
immunohistochemistry: suitable
western blot: suitable
NCBI accession no.
UniProt accession no.
shipped in
dry ice
target post-translational modification
unmodified
Gene Information
human ... SLC32A1(140679)
General description
Vesicular GABA Transporter (unc-47) homologue is a 525 amino acid long protein belonging to the family of neurotransmitter transporter (1). VGAT functions in the uptake of GABA and glycine into synaptic vesicles. Since the protein is bound to synaptic vesicles, VGAT antibodies are excellent markers of presynaptic GABAergic and glycinergic neurons.
Specificity
Antibody cross-reactivity with various species is not known. The immunogen sequence is 100% conserved in mouse, human and monkey.
Specific for Vesicular GABA Transporter (VGAT). Vesicular GABA Transporter (unc-47) homologue is a 525 amino acid long protein belonging to the family of neurotransmitter transporter. The immunogen peptide shows no significant homology with other known proteins.
Immunogen
A 17 amino acid peptide sequence near the carboxy terminal region of rat VGAT (McIntire, S., 1997).
Application
Immunohistochemistry/Immunofluorescence:
2-20 µg/mL of a previous lot was used on paraformaldehyde fixed sections.
ELISA:
1:10,000 – 1:100,000 dilution of a previous lot was used on ELISA, using 50-100 ng of control peptide (Cat. No. AG379) per well.
Western blot:
1-10 µg/mL (Chemiluminescence technique) The expected molecular weight is approximately 55-60 kDa.
Optimal working dilutions must be determined by the end user.
2-20 µg/mL of a previous lot was used on paraformaldehyde fixed sections.
ELISA:
1:10,000 – 1:100,000 dilution of a previous lot was used on ELISA, using 50-100 ng of control peptide (Cat. No. AG379) per well.
Western blot:
1-10 µg/mL (Chemiluminescence technique) The expected molecular weight is approximately 55-60 kDa.
Optimal working dilutions must be determined by the end user.
This Anti-Vesicular GABA Transporter (VGAT) Antibody is validated for use in ELISA, IF, IH, WB for the detection of Vesicular GABA Transporter (VGAT).
Quality
Evaluated by Western Blot on Rat retina lysates.
Western Blot Analysis:
1:500 dilution of this antibody detected Vesicular GABA Transporter on 10μg of rat retina lysates.
Western Blot Analysis:
1:500 dilution of this antibody detected Vesicular GABA Transporter on 10μg of rat retina lysates.
Target description
57 kDa
Physical form
Purified rabbit polyclonal in buffer containing PBS with 0.1% BSA.
Analysis Note
Control
Brain tissue, rat retina lysate
Brain tissue, rat retina lysate
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Legal Information
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
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Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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