AB4330
Anti-DDX4 (Vasa) Antibody
from rabbit, purified by affinity chromatography
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Synonym(s):
DEAD (Asp-Glu-Ala-Asp) box polypeptide 4, probable ATP-dependent RNA helicase DDX4, DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide 4, Vasa homolog, DEAD box protein 4
UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Recommended Products
biological source
rabbit
Quality Level
antibody form
affinity isolated antibody
antibody product type
primary antibodies
clone
polyclonal
purified by
affinity chromatography
species reactivity
rat, human, mouse
technique(s)
immunohistochemistry: suitable (paraffin)
western blot: suitable
NCBI accession no.
UniProt accession no.
shipped in
wet ice
target post-translational modification
unmodified
Gene Information
human ... DDX4(54514)
General description
DDX4 (Vasa or MVH) is a member of the DEAD family of proteins and is a probable ATP-dependent RNA helicase. DDX4 (Vasa) proteins are mostly localized in testicular germ cells in the cytoplasm of spermatocytes. DDX4 (Vasa) is a high-copy extragenic suppressor gene. DDX4 (Vasa) is shown to be significantly decreased in spermatozoa of men that are infertile and may have an important role in male fertility.
Specificity
This antibody recognizes DDX4 (Vasa) at the C-terminus.
Immunogen
Epitope: C-terminus
KLH-conjugated linear peptide corresponding to the C-terminus of mouse DDX4 (Vasa).
Application
Anti-DDX4 (Vasa) Antibody detects level of DDX4 (Vasa) & has been published & validated for use in WB, IH(P).
Immunohistochemistry Analysis: A 1:100-250 dilution from a previous lot detected DDX4 (Vasa) in normal human testis tissue.
Research Category
Stem Cell Research
Stem Cell Research
Research Sub Category
Pluripotent & Early Differentiation
RNA Metabolism & Binding Proteins
Pluripotent & Early Differentiation
RNA Metabolism & Binding Proteins
Quality
Evaluated by Western Blot in mouse testis tissue lysate.
Western Blot Analysis: A 1:500 dilution of this antibody detected DDX4 (Vasa) in 10 µg of mouse testis tissue lysate. Western Blot performed without Tween.
Western Blot Analysis: A 1:500 dilution of this antibody detected DDX4 (Vasa) in 10 µg of mouse testis tissue lysate. Western Blot performed without Tween.
Target description
~76 kDa observed
Physical form
Affinity purified
Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.05% sodium azide.
Storage and Stability
Stable for 1 year at 2-8°C from date of receipt.
Analysis Note
Control
Mouse testis tissue lysate
Mouse testis tissue lysate
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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Irma Virant-Klun et al.
Cells, 8(7) (2019-07-25)
A population of small stem cells with diameters of up to 5 μm resembling very small embryonic-like stem cells (VSELs) were sorted from human embryonic stem cell (hESC) cultures using magnetic-activated cell sorting (MACS) based on the expression of a
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