AB3849-I
Anti-phospho SMAD2 Antibody (Ser465/467)
from rabbit, purified by affinity chromatography
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Synonym(s):
Mothers against decapentaplegic homolog 2, Ser465/467 phosphorylated, hMAD-2, Ser465/467 phosphorylated, hSMAD2, Ser465/467 phosphorylated, JV18-1, Ser465/467 phosphorylated, MAD homolog 2, Ser465/467 phosphorylated, Mad-related protein 2, Ser465/467 pho
UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
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biological source
rabbit
Quality Level
antibody form
affinity isolated antibody
antibody product type
primary antibodies
clone
polyclonal
purified by
affinity chromatography
species reactivity
human
species reactivity (predicted by homology)
zebrafish (based on 100% sequence homology), Xenopus (based on 100% sequence homology), mouse (based on 100% sequence homology), bovine (based on 100% sequence homology), chicken (based on 100% sequence homology), rat (based on 100% sequence homology)
technique(s)
western blot: suitable
NCBI accession no.
UniProt accession no.
shipped in
wet ice
target post-translational modification
phosphorylation (pSer465/pSer467 )
Gene Information
human ... SMAD2(4087)
General description
Mothers against decapentaplegic homolog 2 (UniProt Q15796; also known as hMAD-2, hSMAD2, JV18-1, MAD homolog 2, Mad-related protein 2, Mothers against DPP homolog 2, SMAD 2, SMAD family member 2, Smad2) is encoded by the SMAD2 (also known as MADH2, MADR2) gene (Gene ID 4087) in human. Smad2 is a receptor-regulated intracellular signal transducer and transcriptional modulator activated by transforming growth factor TGF-beta and activin type 1 receptor kinases. In response to TGF-beta signal, smad2 is recruited to the activated TGF-beta receptor through its interaction with the SMAD anchor for receptor activation (SARA) protein, where it is phosphorylated by the TGF-beta receptor. The phosphorylation results in its dissociation from SARA and association with another family member smad4. The smad2-smad4 complex then translocates into the nucleus, where it binds to target promoters and forms a transcription repressor complex with other cofactors. Smad2 can also be phosphorylated by activin type 1 receptor kinase, and thereby transduce activin-dependent signaling events.
Specificity
This antibody detects Smad2 Ser465/467 phosphorylation in TGFbeta-stimulated, but not unstimulated HepG2 cells. The corresponding target phosphoserine residues in the alternatively spliced human Smad2Deltaexon3 (Isoform Short) are pSer435 & pSer437 (UniProt Q15796-2).
Immunogen
Epitope: Phospho-Ser465/467.
KLH-conjugated, synthetic phospho-peptide corresponding to residues surrounding Ser465/467 of human Smad2.
Application
Anti-phospho SMAD2 Antibody (Ser465/467) is an antibody against phospho SMAD2 for use in Western Blotting.
Research Category
Epigenetics & Nuclear Function
Epigenetics & Nuclear Function
Research Sub Category
Transcription Factors
Transcription Factors
Quality
Evaluated by Western Blotting in HepG2 cell lysate.
Western Blotting Analysis: 2.0 µg/mL of this antibody detected Smad2 Ser465/467 phosphorylation in 10 µg lysate from TGFbeta-stimulated, but not unstimulated, HepG2 cells.
Western Blotting Analysis: 2.0 µg/mL of this antibody detected Smad2 Ser465/467 phosphorylation in 10 µg lysate from TGFbeta-stimulated, but not unstimulated, HepG2 cells.
Target description
~53 kDa 52.31 kDa (Isoform Long) and 48.96 kDa (Isoform Short; Smad2Deltaexon3) calculated.
Linkage
Replaces: AB3849
Physical form
Affinity purified
Purified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Storage and Stability
Stable for 1 year at 2-8°C from date of receipt.
Other Notes
Concentration: Please refer to lot specific datasheet.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
recommended
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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