AB3843
Anti-Ezrin/Radixin/Moesin Antibody
Chemicon®, from rabbit
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Synonym(s):
Anti-EBP50, Anti-NHERF, Anti-NHERF-1, Anti-NHERF1
UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Recommended Products
biological source
rabbit
Quality Level
antibody form
affinity purified immunoglobulin
antibody product type
primary antibodies
clone
polyclonal
purified by
affinity chromatography
species reactivity
mouse, monkey, rat, human
manufacturer/tradename
Chemicon®
technique(s)
immunocytochemistry: suitable
immunohistochemistry: suitable (paraffin)
western blot: suitable
NCBI accession no.
UniProt accession no.
shipped in
dry ice
target post-translational modification
unmodified
Gene Information
human ... SLC9A3R1(9368)
Specificity
Recognizes Ezrin/Radixin/Moesin. These proteins function as linkers between the plasma membrane and the actin cytoskeleton, and are involved in cell adhesion, membrane ruffling, and microvilli formation. This antibody does not cross-react with other related proteins such as merlin or band 4.1.
Immunogen
KLH-conjugated, synthetic peptide corresponding to residues surrounding Thr567 of human ezrin.
Application
Detect Ezrin/Radixin/Moesin using this Anti-Ezrin/Radixin/Moesin Antibody validated for use in WB, IC, IH(P).
Research Category
Cell Structure
Cell Structure
Research Sub Category
Adhesion (CAMs)
Adhesion (CAMs)
Western blotting: 1:1,000. The 75 kDa band is Moesin and the 80kDa band is Ezrin and Radixin. (For best results, incubate membrane with diluted antibody in 5% BSA, 1X TBS, 0.1% Tween-20 at 4°C with gentle shaking, overnight)
Immunohistochemistry (Paraffin): 1:50. Positive control tissue is breast carcinoma. Cellular localization is membrane and cytoplasm
Immunocytochemistry (IF): 1:100
Optimal working dilutions must be determined by the end user.
Immunohistochemistry (Paraffin): 1:50. Positive control tissue is breast carcinoma. Cellular localization is membrane and cytoplasm
Immunocytochemistry (IF): 1:100
Optimal working dilutions must be determined by the end user.
Physical form
Protein A and peptide affinity purified immunoglobulin. Liquid in 10 mM sodium HEPES, pH 7.5, 150 mM NaCl, 100 μg/mL BSA, with 50% glycerol.
Storage and Stability
Maintain at -20ºC for 12 months, do not aliquot.
Legal Information
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
WGK
WGK 2
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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