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Key Documents

AB3738-I

Sigma-Aldrich

Anti-Beta Lactamase

from rabbit, purified by affinity chromatography

Synonym(s):

Beta-lactamase TEM, IRT-4, Penicillinase, TEM-1, TEM-16/CAZ-7, TEM-2, TEM-24/CAZ-6, TEM-3, TEM-4, TEM-5, TEM-6, TEM-8/CAZ-2

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100 μL
CN¥2,853.02

CN¥2,853.02

Estimated to ship onMay 12, 2025Details

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100 μL
CN¥2,853.02

About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

CN¥2,853.02

Estimated to ship onMay 12, 2025Details

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Request a Bulk Order

biological source

rabbit

Quality Level

100

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

purified by

affinity chromatography

species reactivity

E. coli

species reactivity (predicted by homology)

all

technique(s)

western blot: suitable

NCBI accession no.

NP_957565

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Anti-Beta Lactamase from rabbit, purified by affinity chromatography

AB3738I

Anti-Beta Lactamase

β-Lactamase from Enterobacter cloacae Type IV, lyophilized powder, 0.2-0.6 units/mg protein (using benzylpenicillin)

P4524

β-Lactamase from Enterobacter cloacae

PrEST Antigen TENM1 Prestige Antigens™ Powered by Atlas Antibodies, buffered aqueous solution

APREST74331

PrEST Antigen TENM1

PrEST Antigen PLXDC1 Prestige Antigens™ Powered by Atlas Antibodies, buffered aqueous solution

APREST72123

PrEST Antigen PLXDC1

Quality Level

100

Quality Level

200

Quality Level

-

Quality Level

-

biological source

rabbit

biological source

bacterial (Enterobacter cloacae)

biological source

-

biological source

-

species reactivity

E. coli

species reactivity

-

species reactivity

-

species reactivity

-

shipped in

ambient

shipped in

-

shipped in

wet ice

shipped in

wet ice

technique(s)

western blot: suitable

technique(s)

enzyme immunoassay: suitable

technique(s)

-

technique(s)

-

General description

Beta-lactamase TEM (EC 3.5.2.6; UniProt P62593; also known as IRT-4, Penicillinase, TEM-1, TEM-16/CAZ-7, TEM-2, TEM-24/CAZ-6, TEM-3, TEM-4, TEM-5, TEM-6, TEM-8/CAZ-2) is encoded by the bla (also known as blaT-3, blaT-4, blaT-5, blaT-6) gene (Gene ID 3244915) in E. coli. TEM-1 belongs to the class-A beta-lactamase family. Beta-lactamases produced by bacteria provide resistance to beta-lactam antibiotics, such as penicillins and cephamycins by breaking open the beta-lactam ring. Over 200 beta-lactamases have been reported that are classified into four main groups (A, B, C, and D) and eight subgroups according to their functional and structural characteristics. The TEM-1 is predominant plasmid-mediated beta-lactamase of gram-negative rods. Six different nucleotide sequences, called blaTEM-1a, blaTEM-1b, blaTEM-1c, blaTEM-1d, blaTEM-1e, and blaTEM-1f, have been described to codify the same TEM-1 beta-lactamase. Ref.: Briñas, L., et al. (2002). Antimicrob. Agents Chemother. 46, 103156-103163.

Specificity

This polyclonal antibody detects E. coli beta-lactamase.

Immunogen

GST-tagged full-length E. coli beta-lactamase, including the signal peptide sequence.

Application

Detect beta-lactamase using this rabbit Anti-Beta Lactamase polyclonal antibody, Cat. No. AB3738-I, validated for use in Western Blotting.

Quality

Evaluated by Western Blotting in lasate from beta-lactamase-transformed E.coli.

Western Blotting Analysis: A 1:100,000 dilution of this antibody detected GST-tagged full-length E. coli beta-lactamase in 0.5 µg of lysate from transformed E. coli.

Target description

31.52/28.91 kDa (with/without signal peptide a.a. 1-23) calculated.

Linkage

Replaces: AB3738

Other Notes

Concentration: Please refer to lot specific datasheet.

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Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 1

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    Nils Peiter et al.
    Life science alliance, 6(1) (2022-11-01)
    Cotranslational insertion of selenocysteine (Sec) proceeds by recoding UGA to a sense codon. This recoding is governed by the Sec insertion sequence (SECIS) element, an RNA structure on the mRNA, but size, location, structure determinants, and mechanism differ for Bacteria

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