AB3420-I
Anti-Surfactant Protein A Antibody
from rabbit
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Synonym(s):
Pulmonary surfactant-associated protein A1, PSP-A, PSPA, SP-A, SP-A1, 35 kDa pulmonary surfactant-associated protein, Alveolar proteinosis protein, Collectin-4
UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Recommended Products
biological source
rabbit
Quality Level
antibody form
purified antibody
antibody product type
primary antibodies
clone
polyclonal
species reactivity
human, mouse
species reactivity (predicted by homology)
primate (based on 100% sequence homology)
technique(s)
immunohistochemistry: suitable (paraffin)
western blot: suitable
NCBI accession no.
UniProt accession no.
shipped in
wet ice
target post-translational modification
unmodified
Gene Information
human ... SFTPA1(653509)
mouse ... Sftpd(20390)
General description
Pulmonary surfactant-associated protein A1 (SP-A1, SFTPA1, PSP-A, SP-A) binds to surfactant phospholipids and contributes to lower the surface tension at the air-liquid interface in the alveoli of the mammalian lung and is essential for normal respiration. There are four main surfactant proteins, known as SP-A, B, C, and D. SP-A was the first surfactant protein to be identified, and is also the most abundant. Its molecular mass varies from 26-38 kDa. It can be found in an open or closed form depending on the other substances present in the system. Calcium ions produce the closed-bouquet form. SP-A is necessary for the production of tubular myelin, a lipid transport structure unique to the lungs. Genetic variations in SP-A are a cause of susceptibility to pulmonary fibrosis idiopathic (IPF) and respiratory distress syndrome in premature infants (RDS).
Specificity
Other homologies: Uniprot shows no homology to Rat.
This antibody recognizes the C-type lectin domain of Surfactant Protein A.
Immunogen
Epitope: C-type lectin domain
KLH-conjugated linear peptide corresponding to the C-type lectin domain of human Surfactant Protein A.
Application
Detect SP-A using this rabbit polyclonal antibody, Anti-Surfactant Protein A Antibody validated for use in western blotting & IHC (Paraffin).
Immunohistochemistry Analysis: A 1:200 dilution from a representative lot detected Surfactant Protein A in human lung tissue.
Research Category
Neuroscience
Neuroscience
Research Sub Category
Developmental Signaling
Developmental Signaling
Quality
Evaluated by Western Blotting in human lung tissue lysate.
Western Blotting Analysis: 0.5 µg/mL of this antibody detected Surfactant Protein A in 10 µg of human lung tissue lysate.
Western Blotting Analysis: 0.5 µg/mL of this antibody detected Surfactant Protein A in 10 µg of human lung tissue lysate.
Target description
~35 kDa observed
Linkage
Replaces: AB3420
Physical form
Format: Purified
Protein A purified
Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Storage and Stability
Stable for 1 year at 2-8°C from date of receipt.
Analysis Note
Control
Human lung tissue lysate
Human lung tissue lysate
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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M Neuhaus et al.
Inflammation research : official journal of the European Histamine Research Society ... [et al.], 68(7), 529-544 (2019-05-16)
Pseudomonas aeruginosa (PA) is the most important opportunistic pathogen in causing nosocomial infections and, furthermore, poses a permanent threat for severe chronic infections in patients with cystic fibrosis or COPD. The transmembrane protein CD26 with dipeptidyl peptidase-4 (DPP4) activity shows
Rosario Barranco et al.
International journal of legal medicine, 133(5), 1461-1467 (2019-06-22)
The diagnosis of drowning is one of the most difficult in forensic medicine. The aim of this study was to analyze pulmonary tissue reactions in death by drowning. In particular, we focused on the immunohistochemical expression of P-selectin, SP-A, HSP70
Sander van Riet et al.
American journal of physiology. Lung cellular and molecular physiology, 322(4), L526-L538 (2022-02-10)
Development of effective treatment strategies for lung tissue destruction as seen in emphysema would greatly benefit from representative human in vitro models of the alveolar compartment. Studying how cellular cross talk and/or (altered) biomechanical cues affect alveolar epithelial function could
Deciphering cell lineage specification of human lung adenocarcinoma with single-cell RNA sequencing.
Zhoufeng Wang et al.
Nature communications, 12(1), 6500-6500 (2021-11-13)
Lung adenocarcinomas (LUAD) arise from precancerous lesions such as atypical adenomatous hyperplasia, which progress into adenocarcinoma in situ and minimally invasive adenocarcinoma, then finally into invasive adenocarcinoma. The cellular heterogeneity and molecular events underlying this stepwise progression remain unclear. In
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