AB3276
Anti-Aquaporin 3 Antibody
Chemicon®, from rabbit
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Synonym(s):
AQP3, GLIP
UNSPSC Code:
12352203
eCl@ss:
32160702
Recommended Products
biological source
rabbit
antibody form
affinity purified immunoglobulin
antibody product type
primary antibodies
clone
polyclonal
purified by
affinity chromatography
species reactivity
rat, mouse
manufacturer/tradename
Chemicon®
technique(s)
immunohistochemistry: suitable
western blot: suitable
NCBI accession no.
UniProt accession no.
shipped in
wet ice
target post-translational modification
unmodified
Gene Information
human ... AQP3(360)
Specificity
Recognizes Aquaporin 3.
SPECIES REACTIVITIES: The epitope specific for Aquaporin 3 is highly homologous in human and sheep (15/18). Reactivity with other species has not yet been tested.
SPECIES REACTIVITIES: The epitope specific for Aquaporin 3 is highly homologous in human and sheep (15/18). Reactivity with other species has not yet been tested.
Immunogen
Highly purified peptide corresponding to residues 275-292 of rat Aquaporin 3 (Accession Number P47862) coupled to KLH.
Application
Anti-Aquaporin 3 Antibody detects level of Aquaporin 3 & has been published & validated for use in WB, IH.
Research Category
Neuroscience
Neuroscience
Research Sub Category
Ion Channels & Transporters
Ion Channels & Transporters
Western blotting: 1:200 on rat kidney membranes. Identifies a 31kDa and 40kDa band. The large band may represent the glycosylated form of the protein.
Immunohistochemistry on mouse kidney sections.
Dilutions should be made using a carrier protein such as BSA (1-3%).
Optimal working dilutions must be determined by the end user.
Immunohistochemistry on mouse kidney sections.
Dilutions should be made using a carrier protein such as BSA (1-3%).
Optimal working dilutions must be determined by the end user.
Physical form
Affinity purified immunoglobulin. Lyophilized from PBS, pH 7.4, containing 1% BSA, 5% sucrose and 0.025% sodium azide. Reconstitute with 200 μL of sterile distilled water. Centrifuge antibody preparation before use (10,000 x g for 5 min).
Storage and Stability
Maintain lyophilized material at -20°C for up to 6 months. After reconstitution maintain at -20°C in undiluted aliquots for up to 6 months. Avoid repeated freeze/thaw cycles.
Analysis Note
Control
Included free of charge with the antibody is 40 μg of control antigen (lyophilized powder). Reconstitute with 100 μL of deionized water. For negative control, preincubate 1 μg of purified peptide with 1 μg of antibody for one hour at room temperature. Optimal concentrations must be determined by the end user.
Included free of charge with the antibody is 40 μg of control antigen (lyophilized powder). Reconstitute with 100 μL of deionized water. For negative control, preincubate 1 μg of purified peptide with 1 μg of antibody for one hour at room temperature. Optimal concentrations must be determined by the end user.
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Legal Information
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Hazard Statements
Precautionary Statements
Hazard Classifications
Aquatic Chronic 3
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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To measure the effects of nicotine on lower urinary tract symptoms (LUTS), bladder blood flow, and the urothelial markers hypoxia-inducible factor 1α (HIF1α), uroplakin III (UPIII), and aquaporin 3 (AQP3). Ten-week-old female Sprague Dawley rats were subcutaneously injected with 2 mg/kg
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Molecular endocrinology (Baltimore, Md.), 20(12), 3412-3420 (2006-09-16)
Nuclear receptors are transcriptional regulators that play important roles in embryonic development and organogenesis. To study the potential roles of nuclear receptors in kidney development, we examined the expression patterns of a subset of nuclear receptors in which specific antibodies
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