AB3256-I
Anti-Luciferase
from rabbit, purified by affinity chromatography
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Synonym(s):
Luciferin 4-monooxygenase
UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Recommended Products
biological source
rabbit
Quality Level
antibody form
affinity isolated antibody
antibody product type
primary antibodies
clone
polyclonal
purified by
affinity chromatography
species reactivity (predicted by homology)
all
packaging
antibody small pack of 25 μL
technique(s)
immunocytochemistry: suitable
western blot: suitable
isotype
IgG
UniProt accession no.
shipped in
ambient
target post-translational modification
unmodified
Gene Information
Photinus pyralis ... Loc116160065(116160065)
General description
Luciferin 4-monooxygenase (UniProt: P08659; also known as EC: 1.13.12.7, Luciferase) is a peroxisomal enzyme that belongs to the ATP-dependent AMP-binding enzyme family. It catalyzes the production of oxyluciferin from luciferin in an ATP and magnesium dependent reaction and releases a photon of light when oxyluciferin returns to the ground state. This reaction proceeds in two steps where the first step involves adenylation of luciferin and in the second step luciferase acts as an oxygenase on adenyl-luciferin to produce oxyluciferin and carbon dioxide, the decay of oxyluciferin producing a photon of light of 562 nm. Luciferase from the firefly is commonly used in reporter assay where the total light emission is proportional to the luciferase activity of the sample.
Specificity
This rabbit polyclonal antibody detects firefly luciferase.
Immunogen
Epitope: unknown
GST-tagged full length recombinant firefly luciferase.
Application
Anti-Luciferase, Cat. No. AB3256-I, is a rabbit polyclonal antibody that detects firefly luciferase and has been tested for use in Immunocytochemistry and Western Blotting.
Immunocytochemistry Analysis: A 1:500 dilution from a representative lot detected Luciferase in HeLa cells transfected with Luciferase expression plasmid.
Research Category
Secondary & Control Antibodies
Secondary & Control Antibodies
Quality
Evaluated by Western Blotting in lysate from HeLa cells transfected with Luciferase expression plasmid.
Western Blotting Analysis: A 1:1,000 dilution of this antibody detected Luciferase in HeLa cells transfected with luciferase expression plasmid.
Western Blotting Analysis: A 1:1,000 dilution of this antibody detected Luciferase in HeLa cells transfected with luciferase expression plasmid.
Target description
~58 kDa observed; 60.75 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
Physical form
Affinity Purified
Purified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Storage and Stability
Stable for 1 year at 2-8°C from date of receipt.
Other Notes
Concentration: Please refer to lot specific datasheet.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
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