AB3092
Anti-Orexin-1 Receptor Antibody
Chemicon®, from rabbit
Synonym(s):
OX1R, Hypocretin Receptor 1
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About This Item
UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
biological source
rabbit
Quality Level
antibody form
affinity purified immunoglobulin
antibody product type
primary antibodies
clone
polyclonal
purified by
affinity chromatography
species reactivity
rat, human, mouse
manufacturer/tradename
Chemicon®
technique(s)
ELISA: suitable
western blot: suitable
NCBI accession no.
UniProt accession no.
shipped in
dry ice
target post-translational modification
unmodified
Gene Information
human ... HCRTR1(3061)
General description
Several peptides associated with feeding behavior have been reported recently. Orexins (Orexin-A and Orexin-B) are a family of hypothalamic neuropeptides selectively expressed in the hypothalamus (Sakurai et al. 1998). Orexin-A and Orexin-B are derived from the same precursor (Prepro-orexin) by proteolytic cleavage. Prepro-orexin is an 130 aa peptide with a putative 33 aa secretory sequence, a hydrophobic core followed by residues with small polar side chains. The expression was detected in brain and to a small extent in testis (Sakurai et al. 1998). These neuropeptides bind and activate two closely related Orexin receptors--G protein coupled receptors (GPCRs) OX1R and OX2R.
Specificity
Recognizes Rat and Human Orexin-1.
Immunogen
A 16 aa peptide near the N-terminus of rat OX1R and a 16 aa peptide near the C-terminus of human OX1R.
Application
Research Category
Neuroscience
Neuroscience
Research Sub Category
CNS Control of Metabolism
Circadian Rhythm & Sleep
CNS Control of Metabolism
Circadian Rhythm & Sleep
This Anti-Orexin-1 Receptor Antibody is validated for use in ELISA, WB for the detection of Orexin-1 Receptor.
Western blot: 1-10 μg/mL using Chemiluminescence technique. Protein runs at 45-48 kDa.
ELISA: 1:10,000 -1:100,000 against 50-100 ng/wel immunogen peptide.
Optimal working dilutions must be determined by end user.
ELISA: 1:10,000 -1:100,000 against 50-100 ng/wel immunogen peptide.
Optimal working dilutions must be determined by end user.
Target description
45-48 kDa
Physical form
Affinity Purified immunoglobulin. Liquid in PBS with 0.1% BSA.
ImmunoAffinity Purified
Storage and Stability
Maintain for 6 months at -20°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Analysis Note
Control
Hypothalamus
Hypothalamus
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Legal Information
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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WGK
WGK 2
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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Xianglong Meng et al.
American journal of translational research, 9(4), 1774-1782 (2017-05-05)
The mesenchymal stem cell (MSC) shows potential in degenerative disc disease (DDD) treatment. However, little is known about the function of heterogeneous nuclear ribonucleoprotein A1 (hnRNPA1) in modulating the chondrogenic differentiation of MSCs. This study aimed to investigate the role
Konstantina Vraka et al.
Biomolecules, 13(4) (2023-05-16)
The orexin system is related to food behavior, energy balance, wakefulness and the reward system. It consists of the neuropeptides orexin A and B, and their receptors, orexin 1 receptor (OX1R) and orexin 2 receptor (OX2R). OX1R has selective affinity
Israt Z Shahid et al.
British journal of pharmacology, 165(7), 2292-2303 (2011-09-29)
The rostral ventrolateral medulla (RVLM) maintains sympathetic nerve activity (SNA), and integrates adaptive reflexes. Orexin A-immunoreactive neurones in the lateral hypothalamus project to the RVLM. Microinjection of orexin A into RVLM increases blood pressure and heart rate. However, the expression
Chung-Wei Hsu et al.
Brain sciences, 11(12) (2021-12-25)
Orexin-A (OX-A) and orexin-B (OX-B) are neuropeptides produced in the hypothalamus. Preclinical and clinical studies suggest that depression and anxiety are associated with the orexin system. In the current study, we used the learned helplessness (LH) animal model of depression
Sheng-Lian Yang et al.
Protein & cell, 3(12), 934-942 (2012-11-20)
Articular cartilage, which is mainly composed of collagen II, enables smooth skeletal movement. Degeneration of collagen II can be caused by various events, such as injury, but degeneration especially increases over the course of normal aging. Unfortunately, the body does
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