AB2281
Anti-Na+K+Cl- Cotransporter 2 Antibody
from rabbit, purified by affinity chromatography
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Synonym(s):
Bumetanide-sensitive sodium-(potassium)-chloride cotransporter 2, Kidney-specific Na-K-Cl symporter, NKCC2A variant A, Na-K-2Cl cotransporter, sodium potassium chloride cotransporter 2, solute carrier family 12 (sodium/potassium/chloride transporters), m
UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Recommended Products
biological source
rabbit
Quality Level
antibody form
affinity isolated antibody
antibody product type
primary antibodies
clone
polyclonal
purified by
affinity chromatography
species reactivity
mouse, rat, human
technique(s)
western blot: suitable
NCBI accession no.
UniProt accession no.
shipped in
wet ice
target post-translational modification
unmodified
Gene Information
human ... SLC12A1(6557)
General description
The sodium-potassium-chloride cotransporter isoform 2 is kidney-specific and is found on the apical membrane of the thick ascending limb of Henle′s loop and the macula densa. It accounts for most of the NaCl resorption with the stoichiometry of 1Na:1K:2Cl and is sensitive to such diuretics as furosemide and bumetanide. Some Bartter-like syndromes result from defects in this gene. SLC12A1 is kidney specific and is essential in the regulation of ionic balance and cell volume. It is a component of an electrically silent transporter system, mediating sodium and chloride reabsorption. Defects in SLC12A1 are the cause of Bartter syndrome type 1, an autosomal recessive disorder characterized by impaired salt reabsorption in the thick ascending loop of Henle with pronounced salt wasting, hypokalemic metabolic alkalosis, and varying degrees of hypercalciuria.
Specificity
This antibody recognizes the cytoplasmic domain of NA+K+CL- Cotransporter 2.
Immunogen
Epitope: Cytoplasmic domain
KLH conjugate followed by NA+K+CL- Cotransporter 2 corresponding to the cytoplasmic domain.
Application
Anti-Na+K+Cl-Cotransporter 2 Antibody detects level of Na+K+Cl-Cotransporter 2 & has been published & validated for use in WB.
Research Category
Neuroscience
Neuroscience
Research Sub Category
Ion Channels & Transporters
Ion Channels & Transporters
Quality
Western Blot Analysis:
1:2,000 dilution of this lot detected NA+K+CL- Cotransporter 2 on 10 µg of rat liver lysate.
1:2,000 dilution of this lot detected NA+K+CL- Cotransporter 2 on 10 µg of rat liver lysate.
Target description
~150 kDa
Physical form
Antigen Affinity Purified
Purified rabbit polyclonal antibody in buffer containing 0.1M Tris-Glycine (pH7.4) 150mM NaCl with 0.05% NaN3.
Storage and Stability
Stable for 1 year at 2-8ºC from date of receipt.
Analysis Note
Control
Rat liver lysates
Rat liver lysates
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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Whey protein hydrolysate increases translocation of GLUT-4 to the plasma membrane independent of insulin in wistar rats.
Morato, PN; Lollo, PC; Moura, CS; Batista, TM; Camargo, RL; Carneiro, EM; Amaya-Farfan, J
Testing null
Meng-Hsuan Lin et al.
JCI insight, 6(20) (2021-09-10)
The prevailing view is that the ClC-Ka chloride channel (mouse Clc-k1) functions in the thin ascending limb to control urine concentration, whereas the ClC-Kb channel (mouse Clc-k2) functions in the thick ascending limb (TAL) to control salt reabsorption. Mutations of
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