AB2257
Anti-Vesicular GABA Transporter Antibody
serum, from rabbit
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GABA and glycine transporter, Solute carrier family 32 member 1, Vesicular GABA transporter, solute carrier family 32 (GABA vesicular transporter), member 1, solute carrier family 32, member 1, vesicular inhibitory amino acid transporter
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biological source
rabbit
Quality Level
antibody form
serum
antibody product type
primary antibodies
clone
polyclonal
species reactivity
rat
species reactivity (predicted by homology)
monkey, human
technique(s)
immunohistochemistry: suitable
western blot: suitable
NCBI accession no.
UniProt accession no.
shipped in
wet ice
target post-translational modification
unmodified
Gene Information
human ... SLC32A1(140679)
mouse ... Slc32A1(22348)
General description
Vesicular GABA transporter (VGAT), the first vesicular amino acid transporter to be molecularly identified, differs in structure from previously cloned vesicular neurotransmitter transporters and defines a novel gene family. VGAT is highly concentrated in the nerve endings of GABAergic neurons in the brain and spinal cord but also in glycinergic nerve endings. In contrast, hippocampal mossy fiber boutons, which are glutamatergic but known to contain GABA, lack VGAT immunoreactivity. Although the great majority of nerve terminals containing GABA or glycine are immunopositive for VGAT, subpopulations of nerve endings rich in GABA or glycine appear to lack the protein. Additional vesicular transporters or alternative modes of release may therefore contribute to the inhibitory neurotransmission mediated by these two amino acids.
Specificity
Cat. # AB2257 recognizes VGAT.
Immunogen
Epitope: C-terminus
KLH conjugated Vesicular GABA Transporter corresponding to the C-terminus.
Application
Anti-Vesicular GABA Transporter Antibody is an antibody against Vesicular GABA Transporter for use in WB, IH.
Immunohistochemistry Analysis:
1:50 - 1:500 dilution from a previous lot detected vesiluar GABA transporter using IHC-Select Detection with HRP-DAB.
1:50 - 1:500 dilution from a previous lot detected vesiluar GABA transporter using IHC-Select Detection with HRP-DAB.
Research Category
Neuroscience
Neuroscience
Research Sub Category
Ion Channels & Transporters
Ion Channels & Transporters
Quality
Routinely evaluated by Western Blot on rat brain lysates.
Western Blot Analysis:
1:500 - 1:1,000 dilution of this lot detected vesicular GABA transporter on 10 µg of rat brain lysate.
Western Blot Analysis:
1:500 - 1:1,000 dilution of this lot detected vesicular GABA transporter on 10 µg of rat brain lysate.
Target description
Approx. 57 kDa
Physical form
Antigen Affinity Purified
Rabbit polyclonal serum in buffer containing 0.1M Tris-Glycine (pH7.4) 15mM NaCl with 0.05% NaN3.
Storage and Stability
Stable for 1 year at 2-8ºC from date of receipt.
Analysis Note
Control
Rat brain lysate.
Rat brain lysate.
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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