AB1928
Anti-Integrin α5 Antibody, CT, Intracellular
serum, Chemicon®
Synonym(s):
CD49e, Fibronectin Receptor alpha Subunit
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All Photos(4)
About This Item
UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
biological source
rabbit
Quality Level
antibody form
serum
antibody product type
primary antibodies
clone
polyclonal
species reactivity
human, chicken, hamster, rat, mouse
manufacturer/tradename
Chemicon®
technique(s)
ELISA: suitable
immunocytochemistry: suitable
immunofluorescence: suitable
immunohistochemistry: suitable
immunoprecipitation (IP): suitable
radioimmunoassay: suitable
western blot: suitable
NCBI accession no.
UniProt accession no.
shipped in
dry ice
target post-translational modification
unmodified
Gene Information
human ... ITGA5(3678)
General description
Integrins are a family of dimeric, transmembrane proteins that mediate cell-cell and extracellular matrix adhesion. Signals transduced by integrins play a role in many biological processes, including cell growth, differentiation, migration and apoptosis. The integrin family is composed of at least 15 alpha and 8 beta subunits that may form over twenty different alpha-beta non-covalently bound dimeric combinations on the cell surface. The alpha subunits all have some homology to each other, as do the beta subunits. Both of the subunits contribute to the binding of the ligand. Integrin alpha subunits contain seven weak sequence repeats in the N-terminal region, which may be important in ligand binding, and have been predicted to fold cooperatively into a single beta-propeller domain with seven beta-sheets. The alpha-3 subunit (CD49c) is highly concentrated in epithelial cells where it strongly adheres to Laminin-5 and Laminin-5 induced rapid adhesion can be blocked by antibodies against the alpha-3 integrin subunit. The alpha-3 subunit exists in two different splice variants, denoted as "A" and "B". The only difference that results from this differential splicing is a total change in the cytoplasmic domain, while the extracellular domain stays the same. Knock-out mice lacking this subunit show prenatal lethality and abnormalities in the kidneys.
Specificity
Antibody recognizes integrin alpha5. Specificity determined by immunoprecipitation from a detergent extract of 35S methionine labeled endothelial cells. No cross-reactivity to alpha1, alpha2, alpha3, alpha4, alpha6 or alphaV. The antibody recognizes an epitope on the cytoplasmic domain of the protein, and it is thus, intracellular.
Reactivity with other species has not been determined.
Immunogen
Synthetic peptide derived from the C-terminal sequence (cytoplasmic domain) of the human alpha5 integrin subunit.
Application
Anti-Integrin α5 Antibody, C-terminus, Intracellular detects level of Integrin α5 & has been published & validated for use in ELISA, IC, IF, IH, IP, RIA & WB.
Western Blot Analysis:
1:1000 dilution of a previous lot was used.
ELISA:
1:500-1:2000 dilution of a previous lot was used in ELISA.
Immunoprecipitation:
Recommended use of 5 μL of antibody for 5x106 cells.
Immunohistochemistry:
1:1000 dilution of a previous lot was used in tissue staining; suggested for use on acetone fixed tissue only.
Immunocytochemistry:
1:1000 of a previous lot was used in immunocytochemistry.
Optimal working dilutions must be determined by the end user.
1:1000 dilution of a previous lot was used.
ELISA:
1:500-1:2000 dilution of a previous lot was used in ELISA.
Immunoprecipitation:
Recommended use of 5 μL of antibody for 5x106 cells.
Immunohistochemistry:
1:1000 dilution of a previous lot was used in tissue staining; suggested for use on acetone fixed tissue only.
Immunocytochemistry:
1:1000 of a previous lot was used in immunocytochemistry.
Optimal working dilutions must be determined by the end user.
Quality
Routinely evaluated by Western Blot on HUVEC lysates.
Western Blot Analysis: 1:1000 dilution of this lot detected integrin alpha 5 on 10 μg of HUVEC lysates.
Western Blot Analysis: 1:1000 dilution of this lot detected integrin alpha 5 on 10 μg of HUVEC lysates.
Target description
114 kDa
Physical form
Rabbit polyclonal antiserum in buffer containing 0.05% sodium azide.
Analysis Note
Control
Mouse 3T3 fibroblasts Skin (Basement Membrane).
Mouse 3T3 fibroblasts Skin (Basement Membrane).
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Legal Information
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
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WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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Mary Ann Stepp et al.
Molecular carcinogenesis, 49(4), 363-373 (2010-01-19)
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S Khan et al.
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Collagen VI is a basement membrane component that regulates epithelial cell-fibronectin interactions.
Groulx, JF; Gagne, D; Benoit, YD; Martel, D; Basora, N; Beaulieu, JF
Matrix Biology null
Katsuyuki Nakamura et al.
Aging cell, 13(1), 175-184 (2013-11-20)
Aging causes phenotypic changes in skeletal muscle progenitor cells (Skm-PCs), such as reduced myogenesis and increased adipogenesis due to alterations in their environment or niche. Secreted protein acidic and rich in cysteine (SPARC), which is secreted into the niche of
Vincent F Fiore et al.
The Journal of cell biology, 211(1), 173-190 (2015-10-16)
Progressive fibrosis is characterized by excessive deposition of extracellular matrix (ECM), resulting in gross alterations in tissue mechanics. Changes in tissue mechanics can further augment scar deposition through fibroblast mechanotransduction. In idiopathic pulmonary fibrosis, a fatal form of progressive lung
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