AB1879
Anti-Caspase 8 Antibody
Chemicon®, from rabbit
Synonym(s):
MACH1, FLICE
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About This Item
UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
biological source
rabbit
antibody form
affinity purified immunoglobulin
antibody product type
primary antibodies
clone
polyclonal
purified by
affinity chromatography
species reactivity
monkey, bovine, rat, human, mouse
manufacturer/tradename
Chemicon®
technique(s)
western blot: suitable
NCBI accession no.
UniProt accession no.
shipped in
wet ice
target post-translational modification
unmodified
Gene Information
human ... CASP8(841)
mouse ... Casp8(12370)
General description
Caspase-8 is a 55 kDa cytosolic protein that is synthesized as an inactive proenzyme. Activation of caspase-8 involves a two-step proteolysis: the cleavage of caspase-8 to generate a 43 kDa fragment and a 12 kDa which is further processed to 10 kDa, and then the p43 is then cleaved to yield p26 and the release of the active site containing p18.
Specificity
AB1879 detects 55 and 43 kDa proteins, corresponding to the proform and large subunit of caspase-8. The antibody also detects human recombinant caspase-8 protein on SDS-PAGE immunoblots.
Immunogen
recombinant, full length, purified human caspase 8
Application
Research Category
Apoptosis & Cancer
Metabolism
Apoptosis & Cancer
Metabolism
Research Sub Category
Caspases
Enzymes & Biochemistry
Caspases
Enzymes & Biochemistry
This Anti-Caspase 8 Antibody is validated for use in WB for the detection of Caspase 8.
Western blot: 0.5 - 4 μg/mL
Optimal working dilutions must be determined by end user.
Optimal working dilutions must be determined by end user.
Target description
55 and 43 kDa
Linkage
Replaces: 04-573; 04-574
Physical form
ImmunoAffinity Purified
Liquid in PBS, pH 7.2, containing 30% glycerol, 0.5% BSA and 0.01% thiomerosal. Affinity Purified immunoglobulin.
Storage and Stability
Maintain for 1 year at -20°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Analysis Note
Control
Skin/Tumor, Embryo
Skin/Tumor, Embryo
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Legal Information
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
For U.S. Customers: Contains mercury; Do not place in trash - dispose according to local, state, or federal laws.
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Description
Pricing
Storage Class Code
10 - Combustible liquids
WGK
WGK 2
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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