AB15894
Anti-Tbr2 Antibody
from chicken, purified by affinity chromatography
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Eomesodermin homolog, T-box brain protein 2, T-brain-2, TBR-2, Tbr2
Recommended Products
biological source
chicken
Quality Level
antibody form
affinity isolated antibody
antibody product type
primary antibodies
clone
polyclonal
purified by
affinity chromatography
species reactivity
mouse
species reactivity (predicted by homology)
human, rat
technique(s)
immunohistochemistry: suitable
western blot: suitable
isotype
IgY
NCBI accession no.
UniProt accession no.
shipped in
wet ice
target post-translational modification
unmodified
Gene Information
human ... EOMES(8320)
General description
Eomesodermin homolog (UniProt: O54839; also known as T-box brain protein 2, T-brain-2, TBR-2, Tbr2) is encoded by the Eomes (also known as Tbr2) gene (Gene ID: 13813) in murine species. T-box genes are a family of transcription factors have a highly conserved DNA binding domain named the T-domain. Tbr2 functions as a transcriptional activator that plays a crucial role during gastrulation, mesodermal specification, and limb patterning. It plays a role in brain development being required for the specification and the proliferation of the intermediate progenitor cells and their progeny in the cerebral cortex. It is also shown to be involved in the differentiation of CD8+ T-cells during immune response regulating the expression of lytic effector genes. Its DNA-binding region is localized to amino acids 278-458. Conditional inactivation of Tbr2 during early brain development is known to cause microcephaly and severe behavior deficits. Two isoforms of Tbr2 have been described that are produced by alternative splicing. Isoform 2 lacks amino acids 463-481. (Ref.: Bulfone, A., et al. (1999). Mech. Dev. 84 (1-2); 133-138).
Specificity
This chicken polyclonal antibody specifically detects T-box brain protein 2 (Tbr2). It targets an epitope within 20 amino acids from the C-terminal region.
Immunogen
Epitope: Transcription Activation Domain
KLH-conjugated linear peptide corresponding to 20 amino acids from the C-terminal region of mouse T-box brain protein 2 (Tbr2).
Application
Anti-Tbr2, Cat. No. AB15894, is a chicken polyclonal antibody that detects Tbr2 and is tested for use in Western Blotting and Immunohistochemistry (Paraffin).
Research Category
Neuroscience
Neuroscience
Research Sub Category
Developmental Neuroscience
Developmental Neuroscience
Tested Applications
- Immunohistochemistry (Paraffin) Analysis: A 1:1,000 dilution from a representative lot detected Tbr2 in Mouse brain tissue sections.
- Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.
Quality
Evaluated by Western Blotting in E13-E14 Mouse brain tissue lysate.
Western Blotting Analysis: A 1:1,000 dilution of this antibody detected Tbr2 in E13-E14 Mouse brain tissue lysate.
Western Blotting Analysis: A 1:1,000 dilution of this antibody detected Tbr2 in E13-E14 Mouse brain tissue lysate.
Target description
~72 and 74 kDa observed; 74.8 kDa calculated.. Uncharacterized bands may be observed in some lysate(s).
Physical form
Affinity purified
Purified chicken polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Storage and Stability
Recommended storage: +2°C to +8°C.
Analysis Note
Control
E13-14 mouse brain lysate
E13-14 mouse brain lysate
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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