AB1588
Anti-Vesicular Acetylcholine Transporter Antibody
Chemicon®, from guinea pig
Synonym(s):
VAChT
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About This Item
UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Recommended Products
biological source
guinea pig
antibody product type
primary antibodies
clone
polyclonal
purified by
affinity chromatography
species reactivity
rat
manufacturer/tradename
Chemicon®
technique(s)
immunohistochemistry: suitable
western blot: suitable
NCBI accession no.
UniProt accession no.
shipped in
wet ice
Specificity
Vesicular Acetylcholine Transporter (VAChT). By Western blot the antibody reacted with a single band in rat brain homogenates. This band corresponded to the size of the predicted rVAChT. There was no staining of blots incubated with the antibody that had been preabsorbed with the peptide used for antibody production. By immunohistochemistry the staining was strong in the inner retina where cholinergic processes are located and weak in cholinergic cell bodies. There was no background staining noted. In the CNS staining was strong in fibers and stains cholinergic cell bodies well.
Immunogen
Synthetic peptide corresponding to the C-terminus of the predicted rat VAChT protein.
Application
Immunohistochemistry: 1:500-1:1,500 on 4% paraformaldehyde fixed tissue. Sections were cut at 15 μm with a cryostat or 30 μm with a sliding microtome.
Immunoblotting
Blocking peptide available (Catalog Number AG223).
Optimal working dilutions must be determined by the end user.
Immunoblotting
Blocking peptide available (Catalog Number AG223).
Optimal working dilutions must be determined by the end user.
Research Category
Neuroscience
Neuroscience
Research Sub Category
Ion Channels & Transporters
Neuronal & Glial Markers
Ion Channels & Transporters
Neuronal & Glial Markers
This Anti-Vesicular Acetylcholine Transporter Antibody is validated for use in IH, WB for the detection of Vesicular Acetylcholine Transporter.
Physical form
Affinity purified immunoglobulin. Lyophilized from 0.1 M PBS with 1.0% BSA and 0.1% sodium azide. Reconstitute with 100 μL of sterile distilled water.
Storage and Stability
Maintain lyophilized material at 2-8°C for up to 12 months. Maintain reconstituted material at -20°C in undiluted aliquots for up to 6 months. Avoid repeated freeze/thaw cycles.
Legal Information
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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