Recommended Products
biological source
sheep
Quality Level
antibody form
serum
antibody product type
primary antibodies
clone
polyclonal
species reactivity
human, sheep, eukaryotes, rabbit
manufacturer/tradename
Chemicon®
technique(s)
immunohistochemistry: suitable
western blot: suitable
NCBI accession no.
UniProt accession no.
shipped in
wet ice
target post-translational modification
unmodified
Gene Information
human ... UBAP2(55833)
Specificity
Recognizes both free ubiquitin and ubiquitin-protein conjugates on Western blots. Labels tangles and Lewy bodies in paraffin or frozen human brain sections.
Immunogen
Ubiquitin from bovine red blood cells coupled to chicken gamma globulin.
Application
Detect Ubiquitin using this Anti-Ubiquitin Antibody validated for use in WB, IH.
Immunohistochemistry: 1:2,000-1:5,000.
Immunoblotting: 1:1,000-1:2,000 (depending on detection system used).
Optimal working dilutions must be determined by end user.
Immunoblotting: 1:1,000-1:2,000 (depending on detection system used).
Optimal working dilutions must be determined by end user.
Research Category
Protein Trafficking
Protein Trafficking
Research Sub Category
Ubiquitin & Ubiquitin Metabolism
Ubiquitin & Ubiquitin Metabolism
Linkage
Replaces: 04-454
Physical form
Sheep serum. Lyophilized, no preservatives. Reconstitute with 100 mL of sterile distilled water. Centrifuge to remove any residue.
Storage and Stability
Maintain at -20 to -70°C in undiluted aliquots for up to 12 months. Avoid repeated freeze/thaw cycles. Glycerol (1:1) can be added for additional stability.
Legal Information
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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Xiao-Wen Lin et al.
PloS one, 8(6), e67633-e67633 (2013-06-27)
Toll-like receptors (TLRs) play a pivotal role in the defense against invading pathogens by detecting pathogen-associated molecular patterns (PAMPs). TLR4 recognizes lipopolysaccharides (LPS) in the cell walls of Gram-negative bacteria, resulting in the induction and secretion of proinflammatory cytokines such
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