All Photos(1)
Synonym(s):
G0/G1 Switch Regulatory Protein 7
UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Recommended Products
biological source
sheep
Quality Level
antibody form
purified immunoglobulin
antibody product type
primary antibodies
clone
polyclonal
species reactivity
rat
manufacturer/tradename
Chemicon®
technique(s)
immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable
western blot: suitable
NCBI accession no.
UniProt accession no.
shipped in
wet ice
target post-translational modification
unmodified
Gene Information
human ... FOS(2353)
General description
c-fos is a member of a family of immediate early gene (IEG) transcription factors (other members include Jun and Egr-1) also identified as proto-oncogenes. The basal expression of c-fos and other IEGs is typically low but increases relatively quickly and often dramatically (15-30 min for mRNA; 30-60 min for protein) in response to changes in cellular activity typically caused by external stimuli, such as stress or neuronal activation. Thus, these IEGs are being used to indicate specific activity driven cellular activation including the identification of neurons activated by various growth factors, neuroactive drugs, and correlated changes in behavioral or physiological states.
Specificity
Recognizes c-fos.
Immunogen
Synthetic c-fos peptide (residues Ser.Gly.Phe.Asn.Ala.Asp.Tyr.Glu.Ala. Ser.Ser.Ser.Arg.Cys) corresponding to amino acids 4-17 of human Fos, conjugated to bovine thyroglobulin.
Application
Immunohistochemistry: 1:500-1:4000, 2-8°C 1-3 days. Stains both fresh frozen and paraffin embedded tissue samples by indirect immunofluorescence and immunoperoxidase.
Recommended fixation is 4% PFA with 0.5% picric acid {Li et al 1999}; triton X-100 pretreatment in the block only; antigen retrieval for paraffin sections is recommended. Microwave citric acid treatment suggested.
Western blot: 10 μg/mL. Detects a band at approximately 55 kDa.
Optimal working dilutions must be determined by the end user.
Recommended fixation is 4% PFA with 0.5% picric acid {Li et al 1999}; triton X-100 pretreatment in the block only; antigen retrieval for paraffin sections is recommended. Microwave citric acid treatment suggested.
Western blot: 10 μg/mL. Detects a band at approximately 55 kDa.
Optimal working dilutions must be determined by the end user.
Research Category
Epigenetics & Nuclear Function
Epigenetics & Nuclear Function
Research Sub Category
Transcription Factors
Transcription Factors
Use Anti-c-Fos Antibody (Sheep Polyclonal Antibody) validated in IHC, IHC(P), WB to detect c-Fos also known as G0/G1 Switch Regulatory Protein 7.
Target description
55 kDa
Physical form
Ammonium Sulfate Precipitation
Format: Purified
Purified immunoglobulin. Lyophilized, no preservatives. Reconstitute with 500 μL of sterile distilled water (1 mg/mL).
Storage and Stability
Maintain lyophilized material at -20 to -70°C for up to 12 months. After reconstitution maintain at -20°C in undiluted aliquots for up to one year.
Avoid repeated freeze/thaw cycles.
During shipment, small volumes of product will occasionally become entrapped in the seal of the product vial. For products with volumes of 200μL or less, we recommend gently tapping the vial on a hard surface or briefly centrifuging the vial in a tabletop centrifuge to dislodge any liquid in the container′s cap.
Avoid repeated freeze/thaw cycles.
During shipment, small volumes of product will occasionally become entrapped in the seal of the product vial. For products with volumes of 200μL or less, we recommend gently tapping the vial on a hard surface or briefly centrifuging the vial in a tabletop centrifuge to dislodge any liquid in the container′s cap.
Analysis Note
Control
Widely expressed
Widely expressed
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Legal Information
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
recommended
Product No.
Description
Pricing
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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