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Synonym(s):
N-methyl D-aspartate receptor subtype 2B, N-methyl-D-aspartate receptor subunit 2B, N-methyl-D-aspartate receptor subunit 3, glutamate receptor subunit epsilon-2, glutamate receptor. ionotropic, N-methyl D-aspartate 2B, Glutamate
UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Recommended Products
biological source
rabbit
Quality Level
antibody form
affinity isolated antibody
antibody product type
primary antibodies
clone
polyclonal
species reactivity
mouse, rat, human
manufacturer/tradename
Chemicon®
technique(s)
immunohistochemistry: suitable
immunoprecipitation (IP): suitable
western blot: suitable
NCBI accession no.
UniProt accession no.
shipped in
dry ice
target post-translational modification
unmodified
General description
NMDA receptors are a class of ionotropic glutamate receptors. NMDA receptor channel has been shown to be involved in long term potentiation, an activity dependent increase in the efficiency of synaptic transmission thought to underlie certain kinds of memory and learning. NMDA receptor channels are heteromers composed of the key receptor subunit NMDAR1 (GRIN1) and 1 or more of the 4 NMDAR2 subunits: NMDAR2A (GRIN2A), NMDAR2B (GRIN2B), NMDAR2C (GRIN2C), and NMDAR2D (GRIN2D). GRIN2B may be a candidate gene for the neurodegenerative disorder dentato-rubro-pallidoluysian atrophy (DRPLA).
Properties of NMDAR include modulation by glycine, inhibition by Zn2+, voltage dependent Mg2+ blockade and high Ca2+ permeability. The involvement of NMDAR in the CNS has become a focus area for neurodegenerative diseases such as Alzheimer′s disease and also epilepsy and ischemic neuronal cell death.
Properties of NMDAR include modulation by glycine, inhibition by Zn2+, voltage dependent Mg2+ blockade and high Ca2+ permeability. The involvement of NMDAR in the CNS has become a focus area for neurodegenerative diseases such as Alzheimer′s disease and also epilepsy and ischemic neuronal cell death.
Specificity
NMDAR2B. Strong and specific immunolabeling of the 180 kDa NMDAR2B subunit of the NMDA receptor. No reactivity to NMDAR2A or NMDAR2C by Western blot. Immunolabeling has been blocked by preadsorption of the antibody with the C-terminal fusion protein that was used to generate the antibody.
Immunogen
C-Terminal Fusion Protein of the NMDAR2B (30 kDa).
Epitope: C-terminus
Application
Anti-NMDAR2B Antibody is an antibody against NMDAR2B for use in IH, IP & WB.
Immunohistochemistry:
1:1,000-1:2,000 dilution of a previous lot was used on immunohistochemistry.
Immunoprecipitation:
3 μL of a previous lot of this antibody (under appropriate conditions) quantitatively immunoprecipitated all NMDAR2B in 200 μg of rat brain.
Western Blot Analysis:
1:100-1:2,000 dilution of a previous lot (NMDAR2B is present in high concentrations in the hippocampus).
Optimal working dilutions must be determined by the end user.
1:1,000-1:2,000 dilution of a previous lot was used on immunohistochemistry.
Immunoprecipitation:
3 μL of a previous lot of this antibody (under appropriate conditions) quantitatively immunoprecipitated all NMDAR2B in 200 μg of rat brain.
Western Blot Analysis:
1:100-1:2,000 dilution of a previous lot (NMDAR2B is present in high concentrations in the hippocampus).
Optimal working dilutions must be determined by the end user.
Research Category
Neuroscience
Neuroscience
Research Sub Category
Neurotransmitters & Receptors
Neurotransmitters & Receptors
Target description
180 kDa
Physical form
Format: Purified
ImmunoAffinity Purified
Purified rabbit polyclonal lyophilized from ammonium bicarbonate (5 mM). Some residual salt may also be present. Reconstitute with 50 μL of PBS. Contains no preservatives.
Storage and Stability
Stable for 1 year at -20ºC from date of receipt.
Analysis Note
Control
Brain tissue, Rat Purkinje cell dendrites, rat hippocampus lysate, HEK 293 cells expressing NR2B.
Brain tissue, Rat Purkinje cell dendrites, rat hippocampus lysate, HEK 293 cells expressing NR2B.
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Legal Information
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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Find documentation for the products that you have recently purchased in the Document Library.
Subunit- and pathway-specific localization of NMDA receptors and scaffolding proteins at ganglion cell synapses in rat retina.
Zhang, J; Diamond, JS
The Journal of Neuroscience null
Josette S Lindahl et al.
Neuropsychopharmacology : official publication of the American College of Neuropsychopharmacology, 29(11), 2065-2073 (2004-05-13)
Phencyclidine (PCP) is a noncompetitive antagonist of the N-methyl-D-aspartate (NMDA) glutamate receptor subtype. It produces transient psychoses in normal individuals and exacerbates psychoses in schizophrenics. When administered to rodents, PCP elicits stereotypic behaviors including unrelenting head swaying, hyperlocomotion, and social
Tzyy-Nan Huang et al.
Journal of psychiatry & neuroscience : JPN, 42(1), 37-47 (2017-02-25)
Human genetic studies have indicated that mutations in calcium/calmodulin-dependent serine protein kinase (CASK) result in X-linked mental retardation and autism-spectrum disorders. We aimed to establish a mouse model to study how Cask regulates mental ability. Because Cask encodes a multidomain
Lei-Lei Liu et al.
Frontiers in cellular neuroscience, 11, 279-279 (2017-09-30)
Recently, a line of evidence has demonstrated that the vertebrate retina possesses a novel retrograde signaling pathway. In this pathway, phototransduction is initiated by the photopigment melanopsin, which is expressed in a small population of retinal ganglion cells. These ganglion
Proteome profiling of s-nitrosylated synaptosomal proteins by isobaric mass tags.
Teodora Stella Wijasa et al.
Journal of neuroscience methods, 291, 95-100 (2017-08-10)
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