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biological source
rabbit
Quality Level
antibody form
serum
antibody product type
primary antibodies
clone
polyclonal
species reactivity
human
manufacturer/tradename
Chemicon®
technique(s)
ELISA: suitable
immunohistochemistry: suitable
immunoprecipitation (IP): suitable
neutralization: suitable
western blot: suitable
NCBI accession no.
UniProt accession no.
shipped in
dry ice
target post-translational modification
unmodified
Gene Information
human ... IFNB1(3456)
General description
Interferon beta is a globular protein consisting of 5 alpha helices with a predicted molecular weight of 20 kDa. Due to glycosylation, however, the observed molecular weight is closer to 25 kDa by SDS-PAGE.
Specificity
Recognizes Human interferon beta.
Immunogen
Recombinant human interferon beta expressed in E. coli
Application
Interferon neutralization: 2 x 10e5 neutralization units/mL where one neutralization unit is the amount of antiserum required to neutralize one unit of human interferon beta (Hu-IFN-b) to a 50% endpoint. Interferon was titrated with the use of the cytopathic effect inhibition assay as described [Rubinstein, S., Familletti, P.C., and Pestka, S. (1981) "Convenient Assay for Interferons," in J. Virol., Vol. 37, 755-758]; [Familletti, P.C., Rubinstein, S., and Pestka, S. (1981) "A Convenient and Rapid Cytopathic Effect Inhibition Assay for Interferon," in Methods of Enzymology, Vol. 78 (S. Pestka, ed.), Academic Press, New York, 387-394]. In this antiviral assay for interferon about 1 unit/mL of interferon is the quantity necessary to produce a cytopathic effect of 50%. The units are determined with respect to the international reference standard for human interferon beta provided by the National Institutes of Health [see Pestka, S. (1986) "Interferon Standards and General Abbreviations," in Methods in Enzymology (S. Pestka, ed.), Academic Press, New York 119, 14-23]. This material is prepared specifically for the effective neutralization of human interferon beta.
Immunohistochemistry
Western blot: 1:5000
Immunoprecipitation
ELISA
Optimal working dilutions must be determined by end user.
Immunohistochemistry
Western blot: 1:5000
Immunoprecipitation
ELISA
Optimal working dilutions must be determined by end user.
Research Category
Inflammation & Immunology
Inflammation & Immunology
Research Sub Category
Cytokines & Cytokine Receptors
Cytokines & Cytokine Receptors
This Anti-Interferon-β Antibody is validated for use in ELISA, IH, IP, NEUT, WB for the detection of Interferon-β.
Target description
20 kDa
Linkage
Replaces: 04-1095
Physical form
Rabbit Serum diluited with PBS. Liquid containing no preservatives.
Unpurified
Storage and Stability
Maintain for 1 year at -20°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Analysis Note
Control
IFN expressing cell lines
IFN expressing cell lines
Legal Information
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
recommended
Product No.
Description
Pricing
WGK
WGK 2
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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