AB1224
Anti-Myeloperoxidase Antibody, human white cell
serum, Chemicon®
Synonym(s):
MPO
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About This Item
UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
biological source
rabbit
Quality Level
antibody form
serum
clone
polyclonal
species reactivity
human
manufacturer/tradename
Chemicon®
technique(s)
ELISA: suitable
western blot: suitable
NCBI accession no.
UniProt accession no.
shipped in
wet ice
target post-translational modification
unmodified
Specificity
Myeloperoxidase (Human White Cells). Monospecific by IEP. Some cross reactivity with Myeloperoxidase from other tissues and species may occur.
Myeloperoxidase (MPO) represents an early appearing and highly reliable intracellular myeloid lineage marker molecule. MPO protein can be detected in a subset of human hematopoietic bone marrow progenitor cells and in granulomonopoietic (GM) cells. However, other myeloid-related cell types such as epidermal Langerhans-type dendritic cells (LC) lack MPO. Therefore, human myeloid progenitors might be subdivided based on MPO protein expression into functional subsets.
Myeloperoxidase (MPO) represents an early appearing and highly reliable intracellular myeloid lineage marker molecule. MPO protein can be detected in a subset of human hematopoietic bone marrow progenitor cells and in granulomonopoietic (GM) cells. However, other myeloid-related cell types such as epidermal Langerhans-type dendritic cells (LC) lack MPO. Therefore, human myeloid progenitors might be subdivided based on MPO protein expression into functional subsets.
Immunogen
Epitope: human white cell
Myeloperoxidase (Human White Cells)
Application
Anti-Myeloperoxidase Antibody, human white cell is an antibody against Myeloperoxidase for use in ELISA & WB.
Immunoblotting: antibody recognizes alpha and beta subunits of MPO. Reduced and denatured extracts of purified MPO produce blots produce 55-60kDa alpha subunits and 12-14kDa beta subunit bands140 Additional bands may be present due to cross reactivity with other myeloperoxidases.
ELISA:
Optimal working dilutions must be determined by end user.
ELISA:
Optimal working dilutions must be determined by end user.
Physical form
Monospecific antiserum purified by delipidation and defibrination. Lyophilized from 0.02 M Potassium Phosphate buffer, 0.15 M Sodium Chloride, pH 7.2, 0.01% Sodium Azide. Reconstitute with 2.0 mL of sterile distilled water.
Storage and Stability
Store unopened vial at 2-8 ºC. Maintain frozen at -20ºC in undiluted aliquots for up to one year after reconstitution. Avoid repeated freeze/thaw cycles.
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Legal Information
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
WGK
WGK 2
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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