Anti-Osteopontin Antibody

Osteopontin is a single-chain polypeptide. It is highly phosphorylated sialoprotein that is a prominent component of the mineralized extracellular matrices of bones and teeth. It is characterized by the presence of a polyaspartic acid sequence and sites of Ser/Thr phosphorylation that mediate hydroxyapatite binding, and a highly conserved RGD motif that mediates cell attachment/signaling. Expression of osteopontin in a variety of tissues indicates a multiplicity of functions that involve one or more of these conserved motifs. While the lack of a clear phenotype in osteopontin "knockout" mice has not established a definitive role for osteopontin in any tissue, recent studies have provided some novel and intriguing insights into the versatility of this enigmatic protein in diverse biological events, including developmental processes, wound healing, immunological responses, tumorigenesis, bone resorption, and calcification. The ability of osteopontin to stimulate cell activity through multiple receptors linked to several interactive signaling pathways can account for much of the functional diversity.

The antibody reacts with the 60 kDa osteopontin protein.

Epitope: aa 75-90

Synthetic peptide corresponding to aa 75-90 of osteopontin protein.

Detect Osteopontin using this Anti-Osteopontin Antibody validated for use in WB, IH.

Research Category
Cell Structure

Research Sub Category
Cytoskeleton

Routinely evaluated by Western Blot on mouse kidney lysate.

Western Blot Analysis:
1:500 - 1:5,000 dilution of this antibody detected osteopontin in 10 μg of mouse kidney lysate.

60 kDa

Serum containing 0.05% sodium azide.

Maintain at -20°C in undiluted aliquots for up to 1 year after date of receipt. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Control
Mouse kidney, COS-1 and human urine lysate

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