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8.52113

Sigma-Aldrich

Fmoc-Asp(biotinyl-PEG)-OH

Novabiochem®

Synonym(s):

Fmoc-Asp(biotinyl-PEG)-OH, N-α-Fmoc-N-γ-(N-biotinyl-3-(2-(2-(3-aminopropyloxy)-ethoxy)-ethoxy)-propyl)-L-asparagine

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25 G
CN¥807.54
100 G
CN¥2,028.06
500 G
CN¥6,183.47
1 KG
CN¥11,052.04
25 KG
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25 KG
CN¥81,199.39

CN¥807.54

Available to ship onApril 07, 2025Details

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25 G
CN¥807.54
100 G
CN¥2,028.06
500 G
CN¥6,183.47
1 KG
CN¥11,052.04
25 KG
inquire
25 KG
CN¥81,199.39

About This Item

Empirical Formula (Hill Notation):
C39H53N5O10S
Molecular Weight:
783.93
UNSPSC Code:
12352209
NACRES:
NA.22

CN¥807.54

Available to ship onApril 07, 2025Details

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Request a Bulk Order

Quality Level

200

product line

Novabiochem®

Assay

≥95.0% (HPLC)
≥98% (TLC)

form

powder

reaction suitability

reaction type: Fmoc solid-phase peptide synthesis

manufacturer/tradename

Novabiochem®

application(s)

peptide synthesis

functional group

biotin

storage temp.

2-8°C

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H3375H7523H6147
HEPES BioPerformance Certified, ≥99.5% (titration), suitable for cell culture

H4034

HEPES

HEPES ≥99.5% (titration)

H3375

HEPES

HEPES BioXtra, pH 5.0-6.5 (1 M in H2O), ≥99.5% (titration)

H7523

HEPES

HEPES BioXtra, suitable for mouse embryo cell culture, ≥99.5% (titration)

H6147

HEPES

technique(s)

cell culture | mammalian: suitable, competitive inhibition ELISA: suitable

technique(s)

cell culture | mammalian: suitable

technique(s)

-

technique(s)

cell culture | embryo: suitable

grade

BioPerformance Certified

grade

-

grade

-

grade

-

Quality Level

400

Quality Level

400

Quality Level

400

Quality Level

400

application(s)

clinical research
diagnostic assay manufacturing
general analytical
life science and biopharma

application(s)

diagnostic assay manufacturing
general analytical
life science and biopharma
pharmaceutical

application(s)

diagnostic assay manufacturing

application(s)

-

form

crystalline powder

form

crystalline powder

form

powder

form

powder

General description

In contrast to Fmoc-Lys(biotin)-OH, this novel biotin-labeled amino acid has excellent solubility in DMF and other solvents used in Fmoc SPPS [1]. The PEG-spacer restricts hindrance between the peptide and avidin, leading to better biotin binding. Furthermore, the hydrophilic nature of the PEG minimizes non-specific interactions that can arise from the spacer group becoming buried in the hydrophobic pocket of proteins.

Associated Protocols and Technical Articles
Biotinylation Reagents for Peptide Synthesis

Literature references

[1] B. Baumeister, et al. (2003) Biopolymers, 71, 339.

Linkage

Replaces: 04-12-1279

Analysis Note

Color (visual): white to slight yellow to beige
Appearance of substance (visual): powder
Identity (IR): passes test
Enantiomeric purity: ≥ 99.5 % (a/a)
Purity (TLC(CMA2)): ≥ 98 %
Assay (HPLC, area%): ≥ 95.0 % (a/a)
Solubility (1 mmole in 2 ml DMF): clearly soluble

To see the solvent systems used for TLC of Novabiochem® products please click here.

Legal Information

Novabiochem is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class Code

11 - Combustible Solids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Regulatory Information

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    Articles

    Biotinylation Reagents

    Biotin-labelled peptides find applications in immunology and histochemistry for affinity purification and receptor localization.

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