Skip to Content
Merck
CN
All Photos(1)

Documents

Safety Information

8.51200

Sigma-Aldrich

DOTA-tris(tert-butyl ester)

Novabiochem®

Sign Into View Organizational & Contract Pricing
All Photos(1)
Synonym(s):
DOTA-tris(tert-butyl ester), Tri-tert-butyl 1,4,7,10-tetraazacyclododecane-1,4,7,10-tetraacetate
Empirical Formula (Hill Notation):
C28H52N4O8
CAS Number:
137076-54-1
Molecular Weight:
572.73
MDL number:
MFCD02259697
UNSPSC Code:
12352108

Quality Level

200

product line

Novabiochem®

form

powder

reaction suitability

reagent type: fluorescent-labelling reagent

manufacturer/tradename

Novabiochem®

mp

88-93 °C

application(s)

peptide synthesis

storage temp.

2-8°C

InChI

1S/C28H52N4O8/c1-26(2,3)38-23(35)19-30-12-10-29(18-22(33)34)11-13-31(20-24(36)39-27(4,5)6)15-17-32(16-14-30)21-25(37)40-28(7,8)9/h10-21H2,1-9H3,(H,33,34)

InChI key

RVUXZXMKYMSWOM-UHFFFAOYSA-N

General description

The most convenient reagent for the labeling of a peptide with DOTA [1]. This reagent has good solubility in DMF and DCM, and its free carboxyl group can be easily activated using uronium or phosphonium coupling reagents, enabling it to be attached to peptide amino groups. Removal of the t-butyl ester groups from the DOTA occurs during the course of the TFA-mediated cleavage reaction. The progress of the reaction should be monitored as the cleavage of these esters is slow, owing to the proximity of the basic ring nitrogens.

Literature references

[1] L. M. De León-Rodrýguez & Z. Kovacs (2008) Bioconjugate Chem, 19, 391.

Analysis Note

Colour (visual): white to grey
Appearance of substance (visual): powder
Identity (IR): passes test
Assay (NMR): ≥ 95.0 %
Identity (NMR): passes test
NMR-spectrum: passes test

Legal Information

Novabiochem is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(C)

Not applicable

Regulatory Information

新产品

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Articles

Peptide Labeling

Chromogenic and fluorogenic derivatives are invaluable tools for biochemistry, having numerous applications in enzymology, protein chemistry, immunology and histochemistry.

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service